Hi, I'm just enquiring what seeding density people use for LHCN-M2 myoblasts and myotubes? Also how long do they leave RNAiMAX in the media for transfection before replacing with fresh media?
Hello, I am trying to run Cygwin terminal but I experience a problem of cyproj-13.dll not found as below. I even checked on Cygwin but it is not there. Kindly help.
11 June 2024 4,502 0 View
I need information on this question
21 May 2024 1,285 1 View
I created a unit cell in CST, and after that I created array from the structure, but my array structures do not behave like the unit cell, a have a DNG material in the unit cell and a SNG in the...
17 May 2024 4,700 6 View
For an assay I am planning to run I require a glucose-free MEM-alpha medium, I've seen papers where researchers state they use glucose-free MEM-alpha but they do not provide a supplier or catalog...
02 May 2024 4,004 0 View
Hi I have following questions regarding iPSC-derived motor neurons: 1. Is it possible to dettached mature motor neurons using accutase and reattach them on coated plates (matrigel, geltrx etc)?...
01 May 2024 3,047 0 View
Can someone please clarify the correct way to calculate LogID50 using the Reed and Muench method. Using the example values below: 10^-1 100% positive 10^-2 100% positive 10^-3 100% positive 10^-4...
01 May 2024 4,113 0 View
Find attached file
30 April 2024 6,886 0 View
In other words, is it simply the nutrient supply that makes soil plants much less effective when they are closer together or does it have to do with the roots interfering with each other. If it's...
16 April 2024 6,718 4 View
Find the attached file.
15 April 2024 6,749 1 View
Hi I`m trying to detect immune response in vitro with my nanoparticle (virus-like particles) incorporating SARS-CoV-2 Spike protein. I inoculated quite a lot of nanoparticle in PBMC and inoculated...
09 April 2024 174 2 View
I am experimenting with cancer and non-cancer cells in a 12-well plate for 4 days with a seeding density 1*10^4/well, however, I noticed that the control group growth rate slows down on D3. Should...
07 August 2024 2,283 2 View
Hi, I'm currently working on a project where I need to plot the atom-projected band structure using GPAW. I've been able to calculate the band structure for my material, but I'm having trouble...
07 August 2024 269 3 View
Hi all, my experimental XPS results shown that my C3N5 sample consists of N-H bond, hence in this case I should incorporate the N-H bond into my DFT modelling. However, I do notice several papers...
07 August 2024 8,414 2 View
Hello, everyone. I have tried to determine carrier motilities of some materials, by Density Functional Theory, using Quantum ESPRESSO. There are a few methods to do it, like a package called...
04 August 2024 8,894 1 View
When the entire neocortex is ablated in rodents, although they are still able to swim, all the limbs move continuously and asynchronously (Vanderwolf 2006; Vanderwolf et al. 1978). Normal animals...
03 August 2024 835 3 View
I would like to perform transfection with the reagent DharmaFECT Duo (Horizon) on the AGS cell line. Could you please inform me of the optimal concentration to use without causing cytotoxicity in...
03 August 2024 3,851 1 View
Dear All, I am trying to transfect a pCDNA3.1 vector containing my gene of interest. The purpose is to figure out the localization of the protein of interest. I have fused the protein with GFP on...
31 July 2024 9,892 4 View
Hello I am trying to create a stable cell line in HEK293 via Lipofectamine 3000 transfection. My plasmid is a CD63-IL10-GFP construct with Puromycin resistance. I am successful with the...
30 July 2024 6,648 1 View
I would like to calculate the formation energy of P2-Na0.67Fe0.5Mn0.5O2 based on DFT, what should I do step by step. Any help would be appreciated. Thanks.
29 July 2024 8,248 2 View
Hello everyone, I am try to understand the differences interaction between insoluble and soluble polymers in water with metal ions and how theit charge density (of metal ions) affect the interaction.
29 July 2024 6,350 3 View