I regularly have low DNA concentrations on the vetigastropod tissues I extract. I have tried fresh tissue and older/museum tissues. We use the Thermo Scientific GeneJET Genomic DNA Purification Kit.
Heating the elution buffer and using less buffer to elute does help, but concentrations are still quite low. Downstream applications are PCR and sequencing.
Super desperate and would appreciate any suggestions on how to increase the yield from extractions!!
Heating the buffer is good but you could try either increasing the elution buffer volume or putting 2 lots of buffer (hot) through the column and then reducu=ing the volume by heating the tube . This may increase yield but leaves the dna more dilute but this is seldom a problem if pcr is the next step
Thanks Paul Rutland ! How should I go about evaporating the volume in the tube? I don't think we have a heat block
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