- The samples are 250 μl serum mixed with 750 μl Trizol
- Isolation by (Chloroform Method )
- Homogenize the sample
- Add 200 μl Chloroform and mix well then incubate on ice for 15 min, then centrifuge to get phase separation (12,000g for 15 min at 4 C )
- Transfer the aqueous phase to fresh tube
- Add 500 μl Isopropanol and mix then incubate on ice for 10 min, then centrifuge (12,000g for 10 min at 4 C )
- This is where RNA is supposed to appear as a white pellet at the bottom of the tube
- The white pellet is not appearing !!
- What are the solutions for this problem ??
Shubhanshu Yadav
Add around 10ul of glycogen with isopropanol with the aqueous phase, incubate at room temperature for 10min and proceed further as usual.
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