20 March 2024 3 7K Report
  • The samples are 250 μl serum mixed with 750 μl Trizol
  • Isolation by (Chloroform Method )
  • Homogenize the sample
  • Add 200 μl Chloroform and mix well then incubate on ice for 15 min, then centrifuge to get phase separation (12,000g for 15 min at 4 C )
  • Transfer the aqueous phase to fresh tube
  • Add 500 μl Isopropanol and mix then incubate on ice for 10 min, then centrifuge (12,000g for 10 min at 4 C )
  • This is where RNA is supposed to appear as a white pellet at the bottom of the tube
  • The white pellet is not appearing !!
  • What are the solutions for this problem ??

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