I have loaded mesoporous silica nanoparticles with a drug and attached them to antibodies but after conjugation with antibodies, the nanoparticles loaded with the drug lose their functionality to kill cancer cells, as if the presence of antibodies block the release of the drug. Anyone has faced the same problem or any idea how to solve it?
Dear Hanieh
1. You'd better to measure the amount of loaded drug after antibody conjugation. Maybe buffers or conditions like pH used for antibody binding caused a burst drug release.
2- If the binding of the antibody is accompanied by unwanted crosslinking, it may negatively affect the efficiency of the system.
3- The hypothesis that you proposed may be true, for example, the binding of a large amount of antibody, in addition to the fact that due to steric hindrance, it cannot be effective in binding to the target cell, but it can interfere with the release of the drug. This is possible both through the creation of steric hindrance and the possibility of interaction between the drug and the antibody.
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