If the sequence of the primers are given as:
Forward: ATATAGACCAATATTCCTGTTAGCA,
Reverse: AAAAATTAGCCGGGCGTAGTGGCG
and size of the PCR product is 2500bp, then what will be the PCR profile for the given reaction
while using Phusion Flash High-Fidelity PCR Master Mix with 0.5uM primer concentration?