Ana-Mariam Kvachadze Yes, there are several free software options for analyzing fluorescence in situ hybridization (FISH) data that are widely used in research. Here are a few notable ones:
1. ImageJ/Fiji: ImageJ is a powerful open-source image processing program that can be used for analyzing FISH images. Fiji is an extended version of ImageJ that comes with many plugins pre-installed, making it more user-friendly for biological imaging.
2. CellProfiler: This is an open-source software designed for quantitative image analysis. It allows users to analyze images from high-throughput FISH experiments, and you can create custom pipelines for different analysis tasks.
3. QuPath: QuPath is an open-source software specifically designed for digital pathology and whole slide image analysis, but it also has capabilities for analyzing FISH images. It offers features for image segmentation, object detection, and quantification.
4. OpenCell: OpenCell is an open-source platform that focuses on the analysis of microscopy images, including FISH. It allows users to apply machine learning techniques to analyze cellular images.
5. Ilastik: Ilastik is a free software tool for interactive image analysis. It includes modules for segmentation, classification, and object tracking, which can be useful for FISH image analysis.
These tools can be quite useful for FISH analysis in impacted articles, depending on the specific requirements of your research. Most of them come with extensive documentation and tutorials to help users get started.