I have used the QIAGEN DNeasy kit to obtain fecal prey DNA from an herbivore and a few of the samples have higher polyphenol levels, which have given a yellowish tint to the final eluted DNA solution.
Should I go ahead with Geneclean (suitable for 200–20 kb) DNA purification from solutions or the traditional ethanol and sodium acetate precipitation to remove the PCR inhibitors in the form of polyphenols and other plant secondary metbolites?
column methods lose a lot of material so I would go for phenol chloroform extraction the salt/alcohol precipitation maybe adding a small amount of glycogen to ensure that all of the dna precipitates. If you do decide to go with dna binding columns then you can increase the yield aby addig twice as much elution buffer and also heating the elution buffer to 70c before using ir
This has happened to me before. I ran the PCR, and it worked fine. However, you have the option to perform another purification with the kit you mentioned or by using AMPURE beads.
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