Dear All

Hi, I am writing to ask your opinion about chance of success designed real time Taqman probe as:

5'- TAC GCC GTA YCT AGC TWY YGC CTG GAC CGT C -3’ as you see in about middle of probe there are 3 degenerate nucleotides (WYY). The main question is should this arrangement make loop or other technical problems. I would be pleased to know probable matters before ordering that.

Best wishes

Mohsen