You would need to be more spicific in the model you are using. Basically the nanodrop gives you the option to select DNA, RNA, Proteins. Yo need to select DNA , then place 2  μL of water (mili Q preferent) select "Blank"  after that place another 2  μL of water to confirm that the measure is 0. Then place 2  μL of your sample. You will get the measurment. Each time you change a sample gentley clean the lense with some smooth tissue. Ask someone in your lab there should be materials for correct use.

This could vary based on the model of Nanodrop you are using, what you're actually trying to quantify, and the source of your DNA. The manual for the ND-1000 is likely a good place to start.

http://www.nanodrop.com/library/nd-1000-v3.7-users-manual-8.5x11.pdf

You would need to be more spicific in the model you are using. Basically the nanodrop gives you the option to select DNA, RNA, Proteins. Yo need to select DNA , then place 2  μL of water (mili Q preferent) select "Blank"  after that place another 2  μL of water to confirm that the measure is 0. Then place 2  μL of your sample. You will get the measurment. Each time you change a sample gentley clean the lense with some smooth tissue. Ask someone in your lab there should be materials for correct use.

Hi,

Pay also attention that the concentration of DNA is calculated by measuring the absorbance at 260 nm but protein contamination also contribute to this A260 measurement. Be sure that the A260/A230 and A260/A280 ratios are above 1.7 - 1.8.

Considering all the technical details has been taken care of, here are some steps I follow to get an accurate measurement using a Nanodrop. I will suggest to take 3-5 reading of the same sample of DNA and get an average of the Absorption value (see below). Then use the equation: A = ε (260) × c × p

ε (260)= extinction coefficient of the DNA sample at 260 nm. This value can be looked up online. If you are trying to get a very accurate measurement you can use the free online software available on  http://www.idtdna.com/ to calculate the value of ε (260) for your sequence.

A=absorbance

p=path length of Nanodrop, usually its is 1 cm.

I hope this information was helpful!