Sometimes, after plating transformed bacterial cells, colonies grow on plate are of different diameters. What are the factors responsible for uneven sizes of colonies on plate?
I possible reason is the some times the transformed cells clump together and the clumped cells will form bigger colonies, One way to avoid that, is to re-suspended the cell pellet few times before plating the cells.
Depends on the overall shape of the colonies. If they are of different shape (or appearance), they are some contamination.
If they otherwise look the same, just with different size, it may be satelite colonies (those tiny ones). So it's better to take the bigger ones, where you know they grew when the antibiotics worked well.
I agree with Tomáš. Unless your transformants seem to have a different color or a very peculiar shape and smell, the variation in size you see is most likely satellite colonies. These are cells that haven't taken up the plasmid, but are growing around colonies that have.
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