Why Internal control is coming earlier than my sample at RT-PCR?

Kiriakos Athanasiadis @Kiriakos-Athanasiadis

25 April 2025 1 8K Report

So my Internal control is coming at 21.5 and my sample to 26.5 Cq. So, i have my standard curves and I make my calculations and is resulting very high value about my sample(Something about 145% lol). Is it possible to have troubleshooting with the DNA Concentration of my sample? Any ideas?

Frederic Lepretre

try to see in nGTEX for example (https://www.gtexportal.org/home/) the relative expression of both genes.

best

fred

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