Why oxidation with peracetic acid engender staining of oxytalan fibers with clastic tissue stains?

Just a quick question above, this oxidation method is mentioned by literature, but no one talked about the principle. Can anyone figure out how the oxidation works on any specific group?

20 December 2020 5,878 1 View

Why does my HPLC filter always generate air bubbles?

I have an old HPLC machine. Every time when I put my filter into 50%:50% water and acetonitrile mixture, my filter always generate bubbles. However, once I change the solvent to 100% acetonitrile,...

08 March 2020 5,587 4 View

What are those clay/silt aggregates? Are those intraclastic clast or flocs?

These deposits were from mud caps of sediment gravity flow deposits. These mud caps are homogenous and no trace fossils can be identified. Can any one help me identify these aggregates? Thank you...

10 September 2018 3,627 3 View

How to measure cell density of bacteria entrapped in hydrogel?

How to determine the cell density or total suspended solids of the bacteria which are entrapped in hydrogel like PVA+Alginate?

26 March 2018 436 3 View

How to compute the band structure of TM mode for 2D photonic crystals by COMSOL?

Can we use the COMSOL to compute the band structure of TM polarization for the 2D photonic crystals? If yes, how to do it? With the "Bandgap Analysis of a Photonic Crystal" in application gallery,...

19 September 2017 1,620 1 View

Why do the same primer real time PCR amplification efficiency is not the same every time?

Why do the same primer real time PCR amplification efficiency is not the same every time?

18 September 2017 8,644 2 View

Flame Retardant materials

I am looking to find commercial available Flame retardant materials so that I can implement them in my plastic pallets which are HDPE-silica composite. The presence of silica do enhance the FR...

09 July 2017 2,900 1 View

"Inflation and the Measurement Problem" written by S. Alexander, D. Jyoti, J. Magueijo. Why their model solve the cosmological measurement problem?

02 May 2017 9,389 3 View

Pathloss model for mobile femtocells?

23 November 2016 9,904 3 View

How to calculate Power of Rayleigh Channel / Rayleigh distributed signal (multipath effect)?

27 October 2016 3,668 5 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Genome-wide gene identification protocol ?

Is there any book chapter/book, webpage or research article available to understand genome-wide gene identification?

28 February 2021 8,095 1 View

Any specific pipeline or parameter to analyse total transcriptomics study of brain tissue samples?

I have two groups of brain samples, control and treated for example. It was total RNA nova seq sequencing. I tried all the available pipeline like: star+rsem+deseq2, Hista+stringtie+cuffdiff,...

27 February 2021 356 6 View

Unknown debris sheets observed in cell culture flasks?

Hi, We culture neural progenitor cells in T175 culture flasks coated with PLO-Laminin. We have successfully grown these cells with our protocol for months. Recently, we observed large sheets...

25 February 2021 3,443 4 View

Can we recognize signs of the "volatility clustering" property of a time series in its time domain plot?

My purpose is to recognize the volatility clustering property without statistical tests such as The Lagrange Multiplier (LM) test.

24 February 2021 4,150 3 View

RNA-seq DEGs downstream analysis and reporting in literature?

I used EdgeR to get the list of DEGs (differentially expressed genes) from my liver RNA-seq experiment. I have done the gene overrepresentation and GSEA based pathway analysis. I am currently...

23 February 2021 8,969 1 View

Could you please suggests a tutorial or paper or any other materials to learn multiple gene expression in TCGA and GTEx databases?

I would like to analyze multiple gene expressions in normal and cancer tissue by using the data in the TCGA and GTEx databases. Please suggest a tutorial or paper showing step by step analysis of...

23 February 2021 9,007 3 View

Are there any databases for gene co-expression or expression pattern clustering?

I am currently working on gene clustering based on co-expression pattern in mouse brain. The problem is I do not have some solid way to test my result. Are there any suggestions for databases...

22 February 2021 4,374 3 View