Hello,Can anyone suggest me protocols to do gene knockout in Klebsiella pneumoniae using CRISPR cas?

06 May 2024 0 3K Report

If anyone can advise me on any protocols which work best for Klebsiella gene knockout are welcome.

Badges
Science topic

More Shan Sts's questions See All

Can anyone explain how to design repair templates in the pSGKP-XX plasmid?

I am trying to use CRISPR and the Lambda red system for gene knockout in Klebsiella. I would like to seek advice on how to design the repair template. If anyone can advise me on this I highly...

05 May 2024 344 0 View

Can anyone help me design gene knockout study for klebsiella ?

I am new to this concept and I need some guidance on designing the primers with restriction site, homologous region, and antibiotic resistance gene casseettee. I am also looking for the suicide...

03 March 2024 8,193 2 View

Hi, could someone please help me with gene knockout in Klebsiella (homologus recombination) and also I need suggestion for choosing the plasmid?

Thanks

28 February 2024 6,562 3 View

Request for scale on political economical and social factors ?

Request for scale on political economical and social factors

13 February 2024 2,755 1 View

Is daratumumab currently a therapeutic strategy for multiple myeloma?

Any other best therapeutic strategy for multiple myeloma

03 January 2024 4,628 3 View

What is 'homogeneity of cellular transduction'?

I'm confused about 'homogeneity of cellular transduction' of AAV. Does this mean AAV can only transduce one certain type of cells after it enters into living organism?

15 May 2023 8,675 3 View

Can you give me some information or some examples of Nestle's behavior of climate change?

I am searching for relative information about Nestle's contribution on climate and the environment. I wonder if the company has ever done some negative or opposite to the goal of protecting the...

14 April 2023 5,901 1 View

Anyone knows any contact from NIBSC.org. "Genetic Reference Panel, for the quantitation of BCR-ABL translocation by RQ-PCR (1st I.S.)"?

Can anyone in the research gate community share contact details of personnel who works in NIBSC.org please. I need some details regarding there "Genetic Reference Panel, for the quantitation of...

04 November 2022 9,177 0 View

How to read the following Graph?

Sanger Sequence trace

29 July 2022 7,725 3 View

How can I get rid of air bubbles from the HPLC pump when mixing mobile phases?

Recently I noticed that air bubbles constantly appeared from my pump and keep clogging my columns. 【Solvents】 Solvent A =sodium acetate buffer (0.05 M, pH 6.0) in water containing 5 mM β-CD...

11 April 2022 5,051 5 View

Weak DAPI staining after immunohistochemistry - how to improve?

After immunohistochemistry of previously fixed in PFA and EtOH and then frozen 20 μm sections of zebrafish brain, DAPI staining is very weak (right) compared to the same sections stained without...

05 August 2024 9,637 2 View

Why did the authors extrapolate a phenotype that they experimentally proved in one bacterial strain across the whole genus of the organism?

I aim to be as skeptical as possible regarding whether a pair of orthologous genes results in the same phenotype in their different but related bacterial organisms under similar environmental...

05 August 2024 6,787 4 View

Why my colony PCR results of my recombinant bacterial not showing any results?

I am performing ligation of the plasmid and a target gene. The steps I have taken are: 1. Double digestion of the plasmid and target gene 2. Ligation of the plasmid with the target gene 3....

05 August 2024 2,570 3 View

How to isolate lymphocytes from mouse spleen?

I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...

04 August 2024 9,913 7 View

What is the acceptable p-value cutoff for GO enrichment analysis ?

I have an RNA-seq data that I have analysed using Limma-voom and have extracted the gene IDs, log2FC and the p-values. At p value < 0.05, I have over 10,000 DEGs, however, when I run the GO...

31 July 2024 225 2 View

Inquiry on Maximum Nucleic Acid Volume for 2.5 mL Liposome Solution?

I am currently working on a project involving liposomes and need to determine the maximum volume of siRNA that can be added to a 2.5 mL liposome solution with a total lipid concentration of 10...

30 July 2024 6,420 1 View

Do some Staphylococcus aureus strains have in vitro antimycobacterial activity ?

Some Staphylococcus aureus strains Inhibit the growth of Mycobacteria in Mueller Hinton Agar medium containing 10% OADC. Do some Staphylococcus aureus strains have in vitro antimycobacterial activity?

29 July 2024 10,023 2 View

MAGeCK Plotting Error: Logarithmic Axis Must Have Positive Limits?

I have been running a MAGeCK test command on the terminal for a CRISPR screen to rank sgRNAs and genes based on the read count tables. However, I get only the plot of the top-ranked genes...

28 July 2024 9,811 1 View

Are these cassettes suitable for expressing PETase mutant in E. coli?

I created two potential gene expression cassettes (constitutive and inducible) for expression of a mutant PETase gene on PeptiCloud using the version tree feature, which allows users to create...

28 July 2024 7,559 1 View

Optimal siRNA Volume for Complexation with Liposomes in a 2.5 mL Solution with 10 mg/mL Lipid Concentration?

How much volume of siRNA can I add to 2.5 mL of liposomes with a total lipid concentration of 10 mg/mL?

27 July 2024 6,920 1 View