Can Achromobacter grow on nitrogen free mannitol broth?

14 March 2024 0 9K Report

We isolated azotobacter from soil for cellulose production but 16srrna identified it's Achromobacter.

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Can we use Spray dried solid lipid nanoparticles for MTT assay?

I have synthesized Quercetin SLNs and spray dried them using Mannitol to avoid drug leakage. However, I need to perform MTT (cytotoxicity). Can I use the powder directly disperse it into media and...

08 January 2021 6,783 3 View

What is the function of sucrose or mannitol in buffer for cell lysis?

In the subcellular isolation experiment, sucrose and mannitol is present in buffers for resuspending cell pellet after centrifugation. For example, Isolation buffer (IB): 220 mM d-mannitol, 70 mM...

28 May 2020 4,608 3 View

Can anyone help identify this unknown bacteria?

I'm having extreme difficulty identifying this bacteria... here are the results of some of the tests I've performed. Any insight is greatly appreciated! TTC Motility: strongly motile Gram stain:...

04 March 2020 2,261 6 View

What is the best ingredients to make ODT which contain arround 100 mg natural extract?

My formulation contains 100 mg natural extract which also use Mannitol and Dextrose as diluent (total amount is 650mg). I have issues during the process, for instance, the granules will stick into...

11 December 2019 4,657 3 View

How to extract mannitol from seaweed using ethanol?

The professors from our university gave us the task to write a literature review on the subject "Extraction and analysis of mannitol in seaweed by HPLC". Despite the fact that we have a...

03 April 2019 9,096 3 View

Looking for a Ph.D where my work makes a difference. ?

Hi Everyone! I am Jyoti Sharma and currently looking for a Ph.D in the area of Microbiology, Genomics and Molecular Biology. I worked on an Azotobacter, soil bacterium as my Master research work...

01 February 2019 7,558 7 View

Does anyone have experience using cryopreservation of plant suspension cells?

I'm having some problems with cryopreservation of BY-2 suspension cells. I followed the protocol from "Initiation, growth and cryopreservation of plant cell suspension cultures, Mustafa et al....

31 December 2018 6,374 1 View

Which 16S reference sequence should I use for Azotobacter chroococcum?

I want to confirm the Azotobacter chroococcum by sequencing it with normal 16s primers, so is there any general sequence available to be used as a reference sequence?

07 August 2018 4,849 0 View

Why the color of XLD and Mannitol agar become lighten ?

The color of my media, such XLD and Mannitol salt agar, become kinda lighten after putting into refrigerator equipping with fans. XLD was originally hazy bright red after heating and...

06 July 2018 6,466 2 View

What this DSC thermogram of lipid nanoparticles indicates? Is it correct thermograpm or having some error?

I have DSC thermogram with me of optimized nanoformulation. What could be the interpritation of this? It consist of drug, lipid, mannitol etc.

04 May 2018 9,330 2 View