Why oxidation with peracetic acid engender staining of oxytalan fibers with clastic tissue stains?

Just a quick question above, this oxidation method is mentioned by literature, but no one talked about the principle. Can anyone figure out how the oxidation works on any specific group?

20 December 2020 5,878 1 View

Why does my HPLC filter always generate air bubbles?

I have an old HPLC machine. Every time when I put my filter into 50%:50% water and acetonitrile mixture, my filter always generate bubbles. However, once I change the solvent to 100% acetonitrile,...

08 March 2020 5,587 4 View

What are those clay/silt aggregates? Are those intraclastic clast or flocs?

These deposits were from mud caps of sediment gravity flow deposits. These mud caps are homogenous and no trace fossils can be identified. Can any one help me identify these aggregates? Thank you...

10 September 2018 3,627 3 View

How to measure cell density of bacteria entrapped in hydrogel?

How to determine the cell density or total suspended solids of the bacteria which are entrapped in hydrogel like PVA+Alginate?

26 March 2018 436 3 View

How to compute the band structure of TM mode for 2D photonic crystals by COMSOL?

Can we use the COMSOL to compute the band structure of TM polarization for the 2D photonic crystals? If yes, how to do it? With the "Bandgap Analysis of a Photonic Crystal" in application gallery,...

19 September 2017 1,620 1 View

Why do the same primer real time PCR amplification efficiency is not the same every time?

Why do the same primer real time PCR amplification efficiency is not the same every time?

18 September 2017 8,644 2 View

Flame Retardant materials

I am looking to find commercial available Flame retardant materials so that I can implement them in my plastic pallets which are HDPE-silica composite. The presence of silica do enhance the FR...

09 July 2017 2,900 1 View

"Inflation and the Measurement Problem" written by S. Alexander, D. Jyoti, J. Magueijo. Why their model solve the cosmological measurement problem?

02 May 2017 9,389 3 View

Pathloss model for mobile femtocells?

23 November 2016 9,904 3 View

How to calculate Power of Rayleigh Channel / Rayleigh distributed signal (multipath effect)?

27 October 2016 3,668 5 View

What are the values needed for gabor filter's parameter for cifar10 data?

Hi, I implemented a code to gabor filter cifar10 data but the images after being filtered and stacked are not clear like the original images. I think the problem is in the way I am using the...

03 March 2021 6,317 1 View

Does anyone have a recommendation for yeast reporter gene plasmid/protocol?

Hi, could anyone recommend a plasmid and/or protocol for reporter gene assay in S. cerevisiae? I want to assess the effect of growth conditions on a transcription factor, so I want to clone it`s...

01 March 2021 210 1 View

Why might my GFP tag be dissociating from my plasmid after transfection?

I have a set of stably transfected cell lines all transfected with plasmids containing GFP tags on the C terminus. During a western blot using anti-GFP antibody, one of my plasmids has dissociated...

01 March 2021 9,310 4 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Expression cloning by using cDNA,do I need to modify the cDNA first?

I am going to have a expression cloning of mammalian gene by using shuttle plasmid to transforming the E.coli However I don't know I should only inserting the Coding sequence ,or I can...

28 February 2021 5,440 3 View

Problem with shRNA transfection. Why are only two plates getting contaminated instead of all?

I transfected my LNCaP-WT cells with 3 shRNA plus their NTC two weeks ago and split two puromycin selected cell plates on Friday last week(Feb 26). I checked for GFP in the cells, and they all...

28 February 2021 4,949 3 View

PUC19 as a transfer plasmid for lentiviral delivery?

Hello, Is it possible to use pUC19 as a transfer vector to be packed in using the second generation viral particles packaging system( pMD2.G; psPAX2 plasmids)? As far as I understand it there is...

28 February 2021 4,868 2 View

Genome-wide gene identification protocol ?

Is there any book chapter/book, webpage or research article available to understand genome-wide gene identification?

28 February 2021 8,095 1 View

Any specific pipeline or parameter to analyse total transcriptomics study of brain tissue samples?

I have two groups of brain samples, control and treated for example. It was total RNA nova seq sequencing. I tried all the available pipeline like: star+rsem+deseq2, Hista+stringtie+cuffdiff,...

27 February 2021 356 6 View

Did anyone ever try using pLVX-TetOne Plasmid with a copGFP as a selection marker instead of Puromycin?

When using a lentiviral vector for inducible expression of genes using the Tet-On system. In the literature, I saw a widely used of pLVX-TetOne-Puro Plasmid, is there a reason why people prefer to...

25 February 2021 1,011 3 View