I´m working with flower cells of a plant that only flourish once a year, can you recommend me a fluorescent dye that can be used in fixed cells that allows me to visualize de membrane of the plant cell vacuole or its lumen?
Because of the pandemic, the original plan that was to visualize vacuoles with fm4-64 (the dye didn´t come on time to the lab) in live cells cannot be carried out, so I need to found a fluorescent dye that works well when used after fixation, preferably with an emission different from red.
If you cannot get a dye at the moment, do you have the regrant to do a transfection to express a plasma m protein and then fixed the cells for experiments? I have done this before for my work. Thank you
Also Alejandra G González-Gutiérrez , In general, I believe if the dyes you use doesn’t stain the cells after The fixation, do you have the ability to fist stain love Cells with the dye and then do the fixation for your studies? I have tried this method in the past and it worked well.
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