I want to use Gold electrode as a reference electrode for electrodeposition of nanostructures. Can i use it and how ??

How to use a Gold electrode as a reference electrode ??

04 January 2021 4,058 4 View

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More than 33 million infections with more than one million deaths worldwide as of 29 Sept 2020, the Wuhan pandemic is a around 10 months long nightmare and showed no signs of improvements. The...

28 September 2020 9,840 6 View

Is it necessary to do heat inactivation after ligation?

We are doing heat inactivation of ligation reaction at 65 C after 12 hours of incubation at 16 C but we are having a problem in getting colonies.

31 August 2020 8,004 3 View

How long we need to wash roots in PBS to reduce the auto fluorescence? Is there any protocol on it?

We have tried dipping roots for 10 min at PBS for three times. But we found that the roots were becoming translucent but they are still fluorescence. So seeking information regarding the time and...

31 August 2020 1,907 3 View

Is it possible to synthesis zero valent iron nanoparticles through autoclaving?

Hi, I need to synthesis phytogenic ZVI nanoparticles. So, I want to know that autoclaving iron salt with plant extract is possible or not.

06 July 2020 3,337 15 View

How to simulate Triboelectric nanogenerator in COMSOL multiphysics software?

I wish to design and fabricate energy harvester using triboelectric nanogenerator.

05 February 2018 299 2 View

How can we resolve two proteins having size difference of 1 kda by electrophoresis? which type of gel should I use ??

Basically I have two proteins of 57 kda mol. weight. One is wild type and another is cleaved one . The size difference of two protein is 1 kda. I have tried different percentage of SDS-PAGE gel...

15 February 2017 3,911 18 View

Hi can anybody please tell me how can we elute the intact protein from SDS-Page gel...?? is there any standard protocol available ??

hi can anybody please tell me how can we elute the intact protein from SDS-Page gel...?? is there any standard protocol available ??

11 May 2016 8,816 3 View

Can I use 3,4-dihydro-2-H pyran and P-TsOH for the protection of hydroxyl group in 4-Hydroxy benzoic acid? What are the other methods for the same?

Hydroxyl group protection of 4-Hydroxy benzoicacid.

03 January 2016 7,128 2 View

How do I perform real sample analysis of ascorbic acid, dopamine and uric acid electrochemically?

While going for real sample analysis of bio-molecules. How do I calculate the Recovery and Standard deviation?

29 June 2015 3,308 1 View

what is the simplest definition of ion exchange capacity

What this unit identifies for the ion exchange capacity ca. 0.92 meq. g-1. I am a newcomer in the membrane area. I just started. I highly appreciate your help

08 May 2021 0 0 View

what is the simplest definition of ion exchange capacity

What this unit identifies for the ion exchange capacity ca. 0.92 meq. g-1. I am a newcomer in the membrane area. I just started. I highly appreciate your help

08 May 2021 0 0 View

How to quantitative analize the data of TNBS assay for gelatin?

Estemeed colleagues, I found some issues regarding the quantification of the data for TNBS assay. There are different protocols on how to perform that but it is clear to me the "fil rouge" that...

02 March 2021 2,616 1 View

How to simultaneously perform multiple biochemical assays?

I am a research scholar working on heavy metal stress on plants. I will be doing biochemical characterisation( protein, carbohydrate, proline, antioxidant enzymes and many more assays) at interval...

01 March 2021 6,999 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

RIA. How to measure?

When you use RIA, with a control the unknown sample with antibodies, you bare in mind the binding sites of the antibodies. However you still need to measure labelled antigen (radioactive) and not...

28 February 2021 2,133 3 View

Dapi fluorescence protocol for colon cell lines ?

28 February 2021 2,534 2 View

Cloudy/high background western blot - can anyone help me?

Hi everyone! I try to detect VDR protein (~48 kDa) using Western Blot. After SDS-PAGE using 10% MP TGX Stain-Free Gel from BioRad I transferred the protein onto an metOH activated PVDF membrane...

23 February 2021 6,266 3 View

I have a time series data of some biochemical studies ( DNA and Chromosomal damage) which I intend to use to further predict into future ?

I have a time series data of some biochemical studies ( DNA and Chromosomal damage) which I intend to use to further predict into future without necessarily conducting the assay for an extended...

23 February 2021 7,842 1 View

What is the standard protocol in doing replicates for C. elegans lifespan assay on solid media (thru worm picking)?

I would like to know what is the standard protocol in the number of worms per plate and number of plates per concentration of extract X in a lifespan assay done in solid media and not automated...

23 February 2021 9,933 1 View