How to increase the signal of the protein standard when performing western blot?

Hi, I'm writing my master's thesis on the characterization of human proteins which I express and purify from E. coli. I'm performing western blot to verify my protein, but the protein standard...

06 October 2020 1,107 5 View

What is your sentiment concern the impact of Covid19 on the main industries in Eu and USA ?

Covid 19 seem to be an huge crisis factor for the economy of several country . Im looking data for a first evaluation on EU and USA economy for to understand the best action for fight this impact .

07 April 2020 5,254 10 View

Tips on interpreting cell morphology and counting mouse primary aortic smooth muscle cells?

Hello, I recently started culturing mouse primary aortic smooth muscle cells (purchased from Cell Biologics) with the media supplied by the same company (5% FBS and a whole lot of other stuff)....

19 March 2020 9,781 3 View

Can someone help with human aortic smooth muscle cells (HASMC)?

Hello, I just started working with human aortic smooth muscle cells (HASMC) for the first time and experienced contamination problems. I purchased Lonza's human smooth muscle cell systems with...

17 February 2019 809 2 View

Isolating high molecular weight DNA from a single feather?

I'm attempting to extract DNA from a single retrix from a small sparrow. I have upwards of 200 samples but only one feather per individual. The feathers have no visible blood spots on them. I've...

14 August 2018 5,190 2 View

Why a gel-liked layer was found on the filter membrane during the ultra-filtration process for polymer nanoparticle?

Recently, I purified a polymer nanoparticle in aqueous phase using ultra-filtration (60 psi). After the filtration process, a gel-liked layer was found on the filter membrane. Dose any one know...

26 June 2018 5,803 3 View

Why an actin band does not appear on gel after PCR for BMM diffrerentiated into osteoclasts?

Hello everyone, I am currently trying to set up at a new lab in order to begin experiments on osteoclasts. Before starting anything, I wanted to make sure that BMMs have indeed differentiated...

17 May 2018 3,308 3 View

How to choose a suitable dialysis tubing or filter membrane?

I need to separate polymer nanoparticle (hydrodynamic radius ~ 110 nm) from poly(ethyleneimine) (MW ~ 750kDa). What MWCO of the dialysis tubing or filter membrane should I choose? How can I make...

22 April 2018 3,966 3 View

Is there any significant difference between Silver and Au as electrodes for polymer thin films dielectric constant measurements?

12 June 2017 3,702 3 View

Does anyone know any questionnaires which measure the use of social media?

I need a questionnaire to measure individuals use of social media e.g. how much they use it etc.

25 October 2016 1,045 3 View

what is the simplest definition of ion exchange capacity

What this unit identifies for the ion exchange capacity ca. 0.92 meq. g-1. I am a newcomer in the membrane area. I just started. I highly appreciate your help

08 May 2021 0 0 View

what is the simplest definition of ion exchange capacity

What this unit identifies for the ion exchange capacity ca. 0.92 meq. g-1. I am a newcomer in the membrane area. I just started. I highly appreciate your help

08 May 2021 0 0 View

Dapi fluorescence protocol for colon cell lines ?

28 February 2021 2,534 2 View

Cloudy/high background western blot - can anyone help me?

Hi everyone! I try to detect VDR protein (~48 kDa) using Western Blot. After SDS-PAGE using 10% MP TGX Stain-Free Gel from BioRad I transferred the protein onto an metOH activated PVDF membrane...

23 February 2021 6,266 3 View

Protocol for membrane extraction E. coli?

Hello! I am interested in determining whether a poly-unsaturated fatty acid I have engineered E. coli to produce is being incorporated into the membrane of the cells or if it is simply a free...

21 February 2021 7,447 2 View

How do you prepare a porous crosslinked Polyvinyl alcohol and Glutaraldehyde membrane or film?

I am trying to prepare a Polyvinyl alcohol membrane for carbondioxide adsorption

08 February 2021 7,493 3 View

Background and spots in Western Blot membrane?

Do you know why my membrane looks like this? I did this western for Anti-hnRNP D/AUF1 antibody ab50692, as primary antibody in 1:1000 overnight at 4c and Anti-rabbit IgG HRP linked antibody...

07 February 2021 5,591 4 View

Acoustic simulation of Membrane?

I have 1 mm thick membrane. This membrane is perforated. I want to simulate this in Acoustic module of comsol. For this ,which type of module I can use in comsol.?

02 February 2021 2,505 6 View

Can i re-incubate a WB membrane with a higher solution of the previously used 1ry ab?

I've incubated my western-blot membrane with both primary and secondary antibodies and i just realized at the end that i've used a lower concentration of my primary antibody for my loading control...

27 January 2021 1,034 3 View

Difficulties purifying Outer Membrane Vesicles using a Sucrose Density Gradient: Unable to detect any protein after this process?

I'm currently trying to purify Outer Membrane Vesicles (OMVs) following their extraction from the bacteria I'm working with. After the initial extraction the concentration of protein seems good...

27 January 2021 6,852 3 View