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I can't get these cells to grow! I have tried a couple different CO2 %ages, FBS concentrations, and tips on whether toget rid of or keep floating cells. Currently, I'm at 15%FBS in DMEM and 5% CO2...
09 September 2017 5,157 1 View
Hello everyone, I am working with mycobacterium abscessus for my thesis. I have one question regarding its growth. Because I am always facing some problems with M.abscessus like sometimes the...
09 September 2017 2,293 2 View
I take maybe two weeks try to solve this problem but it's should be better to ask in this community. My work is to do the agar dilution for my organism Neisseria gonorrhoeae using GC agar base +...
09 September 2017 8,596 7 View
We want to look at TGF-b production in out primary cell cultures from mice but traditionally we have used 10% FBS with our media. The problem is that FBS has TGF-b present normally giving us very...
09 September 2017 6,273 3 View
The Republican candidate Donald Trump received such negative coverage during the Presidential Election campaign last year which was unprecedented in the history of the election whereas Hillary...
05 September 2017 7,014 6 View
I inoculated the cultures on PDA and Oat meal agar media, kept for 4 days in dark and shifted to normal light/dark cycles for another 6 days. But, i am not getting spores? Please advice me how to...
16 August 2017 469 0 View
I have in the past collected at the end of 7 days ~50 million macrophages from 1 mouse. I am in recent weeks getting about 10million macrophages from 1 mouse. Please include precise nature of...
08 August 2017 2,039 1 View
I need an explanation and that which bacteriological madia can be used for cultivation of moulds.
08 August 2017 10,023 2 View
I have froze PMBCs in Complete D-MEM. Should I be thawing these samples in the same media as I froze them or switching to RPMI for the thawing process? L-glutamine is present in RPMI but not in...
08 August 2017 732 4 View
if I am, for example, using BG11 media using "https://utex.org/products/bg-11-medium" how could I get a reference for it. I search papers and couldn't find authentic References for it
08 August 2017 9,361 0 View
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08 August 2017 7,947 0 View
How is citric acid beneficial to microalgae growth?
08 August 2017 1,736 4 View
I am currently culturing human astrocytes in full serum but I read it is recommended to starve the cells before treating them. Could anyone tell me how long these cells would last in Astrocyte...
08 August 2017 4,004 3 View
I am checking the mRNA expression of estrogen receptor along with other genes following estrogen treatment in MCF-7 cells. But I am treating cells with 100nM E2. Is it a very high concentration...
08 August 2017 620 2 View
I have been using an MLR protocol that hasn't turned up any results as far as cell proliferation over the period of 4-5 days. I am using cell strains from Balb/c and B6 mice as stimulators and...
08 August 2017 5,184 1 View
We are trying disperse CNT is both aqueous media using various surfactants but it has poor stability and settles down with time.
08 August 2017 9,085 1 View
it refers to the dilema whether the cable television would get extinct in near future because of digital steaming media? need anwers and suggestions with reasons
08 August 2017 5,800 3 View
Using HUVEC cells: My media has low FBS (2%). I wanted to look at cytokine production after LPS stimulation without removing FBS from the media (because it's possible that it's removal may stress...
08 August 2017 9,068 3 View
To study the release profile of the Eudragit coated humun insulin microspheres for oral delivery
08 August 2017 2,876 1 View
I have used 10%DMSO+90%FBS mixture for freezing cancerous human cells for years with no problem. However, since I began to establish my own laboratory, I am having an isssue that seems to be...
06 August 2017 9,601 6 View
I need conductivity and redox potential measurements over time - fluid media components to be precise. The lesser the invasion of the sensor, the better. If there is a non-invasive design, it...
01 August 2017 8,616 2 View
My cells were supplemented with 0.5% fetal bovine serum (FBS), 10 ng/mL of epidermal growth factor, 5 mg/mL of insulin, 0.5 mg/mL of hydrocortisone, 0.5 mg/mL of epinephrine, 6.5 mg/mL of...
24 July 2017 5,904 6 View
Hi good people, I have got frozen vials of cell lines. I need to know how should I start with this vial for protein extraction? The thawing temperature, the centrifugation speed and all?? Kindly...
23 July 2017 7,812 4 View
Probiotic Food & Beverages
15 July 2017 3,591 5 View