Hi, Jenniffer

I have experience in rodent astrocytes. Astrocytes resist very well starvation. Before every treatment I performed a short starvation of 30 min - 1 hour (medium without FBS) so the FBS doesn't interfere with the treatments. I didn't tested longer starvation periods with primary astrocytes, but astrocyte cell lines resist 24 hours of starvation without problem (no autophagy detected).

I don't recommend to use FBS (1%) along with the treatment, the effect of your treatment could be shadowed by the FBS.

I hope someone with experience in human astrocytes could give you some other tips.

Regards

 Thank You Raul, I tried 6 hours and noticed no visible signs of stress so I will try 24 hours and see. My other cells don't seem troubled with 24 hours of starvation, so I will try it with the astrocytes. I will also try removing the FBS, I agree with you, it may mask the effects of my treatment. Gracias :D

Question: Why do you need long starvation periods? What are you trying to do?

Regards