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Hi,What factors to consider when deciding between alfa- MEM vs. DMEM-HG or DMEM/F12 when growing placenta derived MSC ? why alfa MEM is more better than DMEM-HG or DMEM/F12 . Thanks for your...
05 May 2017 589 0 View
I need company to work on the above asked question..
05 May 2017 3,470 0 View
I used PDA and Swab cotton techniques to isolate Pyricularia but till now not successful. How may I start my job to isolate Pyricularia from rice sample?
05 May 2017 5,014 3 View
Working on shurb, which secrets some phenolic in media so suggest me to control the same
05 May 2017 1,981 3 View
I have 10 micro gram of lyophilized FGFb and 10 micro gram of lyophilized LIF. I need for 20ng/ml (20micro liter/ml) of each components in DMEM/F12 media. in reconstitution they recommend adding...
05 May 2017 3,992 0 View
If Beauveria bassiana is aerobic then why don't we use agitation for media during the growth of culture?
05 May 2017 4,380 0 View
zarrouk medium precipitation..recipe etc.
04 April 2017 1,893 1 View
Nematodes infection in vitro
04 April 2017 8,512 1 View
I want to know how media portrayal of radicalisation has an impact on young muslims in UK.
29 March 2017 1,325 2 View
When I started to study about cell line culture I had a doubt that is why we should using the medium containing serum after when cells start coming off from the culture bottle? What material...
06 March 2017 6,847 1 View
I have cell lines where they like to stick together (not adhering to the plate). Anyway to have the cells in culture not stuck together in order to perform flow? Cells are in RPMI or IMDM media....
03 March 2017 3,349 2 View
03 March 2017 642 2 View
Please let me know
03 March 2017 8,041 4 View
I am preparing a paper about reader's motivation in choosing "his truth" from mainstream or alternative medias. "Reader's truth" means as information, which he considers like real, primary,...
03 March 2017 2,933 0 View
Trypsinization of sheep pox monolayer cells after discarding the media then add trypsin then add media to increase the titer of virus yields,is that application right or not?
03 March 2017 5,284 0 View
I would like to see if my cardiac tissue uses media lipids. Could I use the media to measure intermidiate TCA products? If yes, whick one is the most common?
03 March 2017 9,838 0 View
I saw some publication, there thru ozonation they are converting filter media into biologically active
03 March 2017 8,086 2 View
Hi all. After addition of Retinoic acid into media I can see what seems to be green crystal like structure in the media under the microscope, any ideas?
03 March 2017 451 0 View
Does anyone know how to control the pH of the culture medium using the hypoxia incubator chamber? I'm using nitrogen to make the hypoxic condition and keeping the cells for 72h, but the culture...
02 March 2017 9,669 4 View
Is anyone aware of scholarly research about the (screen)writing of musical numbers in film and television?
26 February 2017 3,068 6 View
I have an experiment where I need to grow E. coli cells in various different concentrations of metals. Therefore, I'm making the media from scratch so I can take metals out completely or add more...
17 February 2017 2,829 1 View
I am about to work on something that requires chitin media but sadly it is too expensive so I was thinking to make my own media like pounding shellwaste into powder, but I think it would affect...
16 February 2017 10,147 1 View
I am working on L929 cell line. After revival of cell line, the flask was not fully confluent but cells are adhered just 40-50% for 3 days, so I trypsined the cells and gave it for spin. After...
04 February 2017 896 4 View
Hi everyone, Last year, I used Essential 8 media (Gibco) with Rock inhibitor (Stemcells Technology) for the first time to recover my clones, and 6/8 of my clones were recovered gradually (forming...
03 February 2017 6,207 2 View