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Questions related to Media
When I started to study about cell line culture I had a doubt that is why we should using the medium containing serum after when cells start coming off from the culture bottle? What material...
06 March 2017 6,877 1 View
I have cell lines where they like to stick together (not adhering to the plate). Anyway to have the cells in culture not stuck together in order to perform flow? Cells are in RPMI or IMDM media....
03 March 2017 3,370 2 View
I want to know how media portrayal of radicalisation has an impact on young muslims in UK.
03 March 2017 657 2 View
Please let me know
03 March 2017 8,054 4 View
I am preparing a paper about reader's motivation in choosing "his truth" from mainstream or alternative medias. "Reader's truth" means as information, which he considers like real, primary,...
03 March 2017 2,948 0 View
Trypsinization of sheep pox monolayer cells after discarding the media then add trypsin then add media to increase the titer of virus yields,is that application right or not?
03 March 2017 5,297 0 View
I would like to see if my cardiac tissue uses media lipids. Could I use the media to measure intermidiate TCA products? If yes, whick one is the most common?
03 March 2017 9,853 0 View
I saw some publication, there thru ozonation they are converting filter media into biologically active
03 March 2017 8,104 2 View
Hi all. After addition of Retinoic acid into media I can see what seems to be green crystal like structure in the media under the microscope, any ideas?
03 March 2017 463 0 View
Does anyone know how to control the pH of the culture medium using the hypoxia incubator chamber? I'm using nitrogen to make the hypoxic condition and keeping the cells for 72h, but the culture...
02 March 2017 9,687 4 View
Is anyone aware of scholarly research about the (screen)writing of musical numbers in film and television?
26 February 2017 3,087 6 View
I need to use antibiotic agar which, say, contains 20ul each of streptomycin, gentamycin and tetracycline. So instead of adding three type of stock during preparation.. I prepare 1ml aliquots...
24 February 2017 7,241 3 View
I have an experiment where I need to grow E. coli cells in various different concentrations of metals. Therefore, I'm making the media from scratch so I can take metals out completely or add more...
17 February 2017 2,841 1 View
I am about to work on something that requires chitin media but sadly it is too expensive so I was thinking to make my own media like pounding shellwaste into powder, but I think it would affect...
16 February 2017 10,165 1 View
I am working on L929 cell line. After revival of cell line, the flask was not fully confluent but cells are adhered just 40-50% for 3 days, so I trypsined the cells and gave it for spin. After...
04 February 2017 909 4 View
Hi everyone, Last year, I used Essential 8 media (Gibco) with Rock inhibitor (Stemcells Technology) for the first time to recover my clones, and 6/8 of my clones were recovered gradually (forming...
03 February 2017 6,224 2 View
Hi, I want to do drought stress using PEG6000, I had tried using normal protocol 1% MS with Plant Agar and 30% peg 6000 and But is was not solidify,and I have also noticed after adding PEG...
02 February 2017 5,148 0 View
I am a beginner in maintaining MIN6 and Beta TC6 cells. I am curious regarding the need for using BME in the culture media for maintaining the cells.
02 February 2017 443 1 View
02 February 2017 6,209 1 View
02 February 2017 7,666 1 View
Is there any company which can have a media having all the required nutrients for the optimum growth of fungi or bacteria?
02 February 2017 1,789 6 View
Has anyone cultured 'raw 264.7(macrophage)' with Leibovitz's L-15 medium? usually, many people culture 'raw 264.7 cell with DMEM But , I don't have Co2 incubator in my lab. So .. Can I use to...
01 February 2017 9,400 0 View
So last night our -80 freezer went down and we didn't realize it until the temperature had reached -25 at which point we put everything in another -80. We had stored all our cell lines in there in...
31 January 2017 2,796 3 View
the role of British media as an instrument of the state.
01 January 2017 8,602 2 View