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Questions related to Media
My clinical isolates are approximately 100 isolates in a total and I need to perform agar dilution at least 7 antibiotics with them. I spent a few week to learn how to do it because no one in my...
13 December 2017 1,865 3 View
Just aCSF media will work or need some additives? Regards
12 December 2017 3,263 3 View
I have heard that between 24 and 48 hours is a good length of time. I plan to add the virus to fresh media with serum and antibiotics, wait 48 hours, and then change out the media. I am performing...
12 December 2017 3,128 1 View
Dung fungi Media Conditions
12 December 2017 3,005 1 View
Hello. I'm trying to isolate bacillus sp. form me-ju. I would like to get bacillus sp. by using selective media. I will appreciate if anyone let me know which media is best for!
12 December 2017 3,335 3 View
12 December 2017 5,040 3 View
Finding consistent literature on incubation temperature - 30°C - but little else on time, pH, and media.
12 December 2017 8,285 1 View
I am planning to do a Netnographic analysis mixed with individual in-depth interviews about women's motivation for posting fitness media on Instagram. I really appreciate your help.
12 December 2017 8,563 1 View
I noticed that some protocols use differentiation media for 2-3 days while others go for 5-7 days before changing to maintenance media. Why is that? Is an adipocyte grown in DM 3 days + MM 4 days...
12 December 2017 791 1 View
Looking for general media or media formulation for thermophilic aerobic/anoxic archaea around neutral pH.
12 December 2017 9,246 2 View
When picking single clones, I use cloning cylinders. A problem which I have faced is that when I use 100 uL of trypsin per cylinder, no cells adhere when directly transferred to a 48-well plate. I...
11 December 2017 9,180 2 View
09 December 2017 2,009 1 View
06 December 2017 880 1 View
I've just joined a project in protein production. We have an issue when growing E.coli in exactly the same conditions, same media, same cell line, same flask type, same incubator etc, some of the...
06 December 2017 2,118 3 View
05 December 2017 1,248 3 View
The treatment that is given to the news of deaths due to external causes can condition new fatal accidents. In the case of traffic accidents there is much more information than in cases of...
30 November 2017 4,967 8 View
I AM TRYING TO ISOLATE C.jejuni BUT AS ITS FROM ENVIRONMENTAL SAMPLE, ITS DIFFICULT. I AM CURRENTLY USING blood free campylobacter broth base AND AGAR FROM HIMEDIA AT 42C
21 November 2017 1,884 8 View
I would like to find an alternative to TSB, with known composition. Thank you so much!
11 November 2017 9,962 2 View
I am working on Okra tissue culture but i am facing problem about okra seed germination , for seed treatment i am using HgCl2 0.1% for 3 minutes and after washing with dd water use 75 %...
11 November 2017 1,773 6 View
11 November 2017 2,306 8 View
In my work I have noticed Leptospira interrogans bunching together when grown in liquid media. I have found references to these bunches being called "brood nests". In the laboratory how can I...
11 November 2017 2,979 0 View
Hi all, I'm growing T-cells with cytokines. I was wondering if there is a quick way to measure how much of the supplemented cytokines the cells are consuming (concentration in media during...
11 November 2017 9,372 3 View
Dear all, I am looking for electron carrier which can carry an electron (charge) from media to Scenedesmus obliquus. The carrier should be passively transported from media through the cell wall....
11 November 2017 3,344 2 View
what is the best broth media for enhancement marine actinomycetes secondary metabolites (Antibiotics) and its components pleas? Thanks
11 November 2017 5,912 4 View