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Questions related to Media
what is the best broth media for enhancement marine actinomycetes secondary metabolites (Antibiotics) and its components pleas? Thanks
11 November 2017 5,828 4 View
I have a problem with agglomeration of ZnO and GaN nanoparticles (nanopowder of 50-nm) in aqua media and alcohols. I am looking forward to know others experience on this issue.
11 November 2017 7,230 4 View
I want to use MES buffer in my microbial fermentation media, The content of buffer will be also used by microbes or it is not usable? It will needs to care about the buffer contents in media also...
11 November 2017 9,827 3 View
I want to analyse the way how Belgium politics uses media during the political campaign. In particular, due the fact that voting in Belgium is mandatory, how politicians use the tv ads or internet...
11 November 2017 7,821 4 View
Hello everybody I would be appreciated if could suggest a practical technique for selective growing medium for to promote growth and sporulation of Glomus intraradices under laboratory conditions.
11 November 2017 5,917 3 View
Chitin agar remains off white to cream before autoclave. But after autoclave it turns brown color. The color of the media should be off white to cream.
06 November 2017 4,234 2 View
Relatively new to splitting cells in tissue culture. I am splitting cells on Monday and Fridays, with a 1:10 split. My current advised protocol (just for amounts) is as follows: 1) Remove...
26 October 2017 1,374 1 View
We just started to culture THP-1 cells and treat them with PMA (100nM) for 72 h. Afterwards we change the media daily as recommended. Does anyone have experiences with serum-free culture in the...
24 October 2017 4,700 1 View
Do you need to reduce surface tension to better dispersion of anti-foam on foam media, and what factors affect surface tension? What factors influence two-phase interaction?
10 October 2017 3,723 13 View
The pictures are on imgur under the following link: https://imgur.com/a/tOL8p Most but not all flasks using the same media have this issue. When I change the culture flask the crystals appear...
10 October 2017 9,401 2 View
10 October 2017 3,794 1 View
Hello everyone. i want to isolate and culture bacteria from infected non vital teeth. predominantly the anaerobes are responsible for the primary infection. my question is can i use BHI media for...
10 October 2017 5,343 1 View
I've been trying to detect nitrite from bacterial cells cultures in which the bacteria had a construct expressing bNOS. Tried with a commercial kit (from sigma. This one also converts nitrate to...
10 October 2017 5,384 2 View
10 October 2017 8,174 1 View
Hi every one, I have a problem in isolating bacteria capable of degrading sulfamethoxazole (SMX). My inoculum is granular activated carbon collected from lab biological activated carbon filter....
10 October 2017 607 2 View
How do i measure watchdog role of print media in content analysis of newspapers? If anybody has this type of study paper please share me?
10 October 2017 2,366 5 View
10 October 2017 7,133 1 View
I've been seeding some human keratinocytes onto different substrates/conditions which demanded different media, from a stock of cells suspended in Keratinocyte Serum-Free Media (KSFM),...
10 October 2017 5,790 3 View
I have been growing my hybridoma cells inGibco Hybridoma SFM . I have cryopresrved them using 92.5% medium (50:50 ratio of fresh to conditioned media) +7.5% DMSO , the viability was 87%. i froze...
10 October 2017 709 1 View
I have attached an image and graphs of the nitrocellulose membrane from the Spot blot assay. My question is regarding the BM8 lanes. From the immunofluorescence analysis it shows that cells in...
10 October 2017 7,545 2 View
I am looking for a media that gives optimum growth of Cellulomonus and Cellvibrio mixtus. It would be of help if anyone can suggest the proper growth condition of the same.. Thanks!!
10 October 2017 225 3 View
I am using Matrigel Corning for cell invasion assay. How can I make a thin Matrigel layer?
21 September 2017 2,104 2 View
Dear all, I am wondering how to evaluate the purity of monocyte derived macrophages (MdM) using flow cytometer. MdM are obtained by adherence isolation, through culture of purified CD14+ monocytes...
09 September 2017 5,318 2 View
The Republican candidate Donald Trump received such negative coverage during the Presidential Election campaign last year which was unprecedented in the history of the election whereas Hillary...
09 September 2017 4,843 6 View