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Questions related to Media
When picking single clones, I use cloning cylinders. A problem which I have faced is that when I use 100 uL of trypsin per cylinder, no cells adhere when directly transferred to a 48-well plate. I...
11 December 2017 9,242 2 View
I noticed that some protocols use differentiation media for 2-3 days while others go for 5-7 days before changing to maintenance media. Why is that? Is an adipocyte grown in DM 3 days + MM 4 days...
09 December 2017 2,100 1 View
I have heard that between 24 and 48 hours is a good length of time. I plan to add the virus to fresh media with serum and antibiotics, wait 48 hours, and then change out the media. I am performing...
06 December 2017 943 1 View
I've just joined a project in protein production. We have an issue when growing E.coli in exactly the same conditions, same media, same cell line, same flask type, same incubator etc, some of the...
06 December 2017 2,203 3 View
Just aCSF media will work or need some additives? Regards
05 December 2017 1,291 3 View
The treatment that is given to the news of deaths due to external causes can condition new fatal accidents. In the case of traffic accidents there is much more information than in cases of...
30 November 2017 5,017 8 View
I AM TRYING TO ISOLATE C.jejuni BUT AS ITS FROM ENVIRONMENTAL SAMPLE, ITS DIFFICULT. I AM CURRENTLY USING blood free campylobacter broth base AND AGAR FROM HIMEDIA AT 42C
21 November 2017 1,930 8 View
I would like to find an alternative to TSB, with known composition. Thank you so much!
11 November 2017 10,020 2 View
I am working on Okra tissue culture but i am facing problem about okra seed germination , for seed treatment i am using HgCl2 0.1% for 3 minutes and after washing with dd water use 75 %...
11 November 2017 1,844 6 View
11 November 2017 2,363 8 View
In my work I have noticed Leptospira interrogans bunching together when grown in liquid media. I have found references to these bunches being called "brood nests". In the laboratory how can I...
11 November 2017 3,043 0 View
Hi all, I'm growing T-cells with cytokines. I was wondering if there is a quick way to measure how much of the supplemented cytokines the cells are consuming (concentration in media during...
11 November 2017 9,441 3 View
Dear all, I am looking for electron carrier which can carry an electron (charge) from media to Scenedesmus obliquus. The carrier should be passively transported from media through the cell wall....
11 November 2017 3,410 2 View
what is the best broth media for enhancement marine actinomycetes secondary metabolites (Antibiotics) and its components pleas? Thanks
11 November 2017 5,969 4 View
I have a problem with agglomeration of ZnO and GaN nanoparticles (nanopowder of 50-nm) in aqua media and alcohols. I am looking forward to know others experience on this issue.
11 November 2017 7,359 4 View
I want to use MES buffer in my microbial fermentation media, The content of buffer will be also used by microbes or it is not usable? It will needs to care about the buffer contents in media also...
11 November 2017 9,986 3 View
I want to analyse the way how Belgium politics uses media during the political campaign. In particular, due the fact that voting in Belgium is mandatory, how politicians use the tv ads or internet...
11 November 2017 7,952 4 View
Hello everybody I would be appreciated if could suggest a practical technique for selective growing medium for to promote growth and sporulation of Glomus intraradices under laboratory conditions.
11 November 2017 6,061 3 View
Chitin agar remains off white to cream before autoclave. But after autoclave it turns brown color. The color of the media should be off white to cream.
06 November 2017 4,357 2 View
Relatively new to splitting cells in tissue culture. I am splitting cells on Monday and Fridays, with a 1:10 split. My current advised protocol (just for amounts) is as follows: 1) Remove...
26 October 2017 1,499 1 View
We just started to culture THP-1 cells and treat them with PMA (100nM) for 72 h. Afterwards we change the media daily as recommended. Does anyone have experiences with serum-free culture in the...
24 October 2017 4,877 1 View
Do you need to reduce surface tension to better dispersion of anti-foam on foam media, and what factors affect surface tension? What factors influence two-phase interaction?
10 October 2017 3,869 13 View
The pictures are on imgur under the following link: https://imgur.com/a/tOL8p Most but not all flasks using the same media have this issue. When I change the culture flask the crystals appear...
10 October 2017 9,527 2 View
10 October 2017 3,929 1 View