Beta value calculation of absolute intracellular calcium concentrations?

Hi all, I am doing calcium imaging experiments in N2a cells and wanted to calculate the absolute calcium concentrations. Can someone help me out in figuring out how to calculate the fluorescence...

24 March 2020 7,974 1 View

What is the significance of 'dark spectral response'?

Can anyone explain me the significance of ''dark spectral response'' or " Dark EQE" of solar cell and how it will help to improve the efficiency of solar cell?

17 April 2019 7,021 6 View

What can I find out by performing DSC on a polymer sample? Also how would I interpret a curve that I obtained?

Metallurgical engineer who has never performed DSC and now I am working with polymers. Am able to do the test on the equipment however I am still facing troubles with interpreting the curve that I...

06 March 2018 7,660 4 View

Which polymer can be in rich in surface when a mixture of two hydrophobic polymers coated on substrate?

"A" is the polymer having low molecular weight and low surface energy. "B" is the polymer with high molecular weight and high surface energy. In a mixture "B" is slightly large concentration than...

31 October 2016 1,742 2 View

How to avoid this warning in Silvaco?

No ionization integrals possible at this bias point. Possibly applied voltage is less than DELTAV in field line calculation or the electrode does not contact any semiconductor material.

20 October 2016 8,761 2 View

How do I select the best primer which does not produce any non specific bands from those I have designed?

Hi! I am currently designing primers for the human UGT1A1 gene using the Primer3 software. However, when I BLAST the primers against the Human RefSeq database, I get significant matches with other...

10 October 2016 7,172 3 View

How to perform enzyme activity assays?

If i want to perform any enzyme activity how to select the substrate concentration in 3 mL reaction mixture? and if there is any donor is present in 3 mL mixture how to select the concentration of...

07 August 2016 8,136 1 View

How to prepare origami competent cells?

Should i need to add antiboitcs ? if so what is the concentrations?

19 July 2016 5,161 2 View

How does the surface roughness on hydrophilic materials change to superhydrophilic surfaces?

Nano/micro roughness causes an increase or decrease in the the contact angle depending on the nature of solid substrates. Trapped air causes an increase in the contact angle for hydrophobic...

29 December 2014 5,341 6 View

Why does the ambipolar diffusion happen in organo metal halide perovskites?

Perovskites are becoming popular materials for solar cells.This is mainly due to the optimal band gap and property of ambipolar diffusion of electrons and holes.

21 December 2014 6,695 4 View

How to grow E.coli on the low salt LB agar plate with 50 or 100ug/ml of Blasticidin?

Hello, I would like to ask how to grow E.coli which resists 50 or 100ug/ml of Blasticidin. I have tried many methods by using low salt Luria-Bertain(LB) agar plate. However, no colonies grow. Let...

01 March 2021 2,027 2 View

Can bacterial suspension be pipetted directly onto petri dish and agar poured onto it?

Hi, recently I encountered a protocol for plating which was not the usual method I have been practicing (pour LB agar onto plate, allow it to solidify, pipette bacterial suspension onto plate,...

28 February 2021 7,292 3 View

Difficulty in cloning of 1kb gene into vector?

I am facing difficulties in cloning a 1kb gene into a vector (pJIT163). I have my gene on interest (GOI) in pUC57 and want to clone in pJIT163 using SalI and BamHI restriction sites. I am getting...

25 February 2021 3,221 7 View

Can cells be reused after using WST 8/ CCK 8 from Sigma Aldrich?

Hi all, Can cells be reused after I have seed them onto 96 well plates and test them with cell counting kit at OD450 (CCK8) at 12 hours, wash them with PBS x3 and add new media and let them...

24 February 2021 5,072 1 View

No title

22 February 2021 9,932 11 View

Is it okay to mention you distributed your qualitrics study link through social media sites?

Just want to be clear and know it is okay to say I have distributed the qualitrics link through social media such as Facebook, Instagram and Twitter to ensure my target audience is reached in my...

21 February 2021 5,143 2 View

What is the minimum number of exosomes/ml to have a good yield of total RNA for Illumina sequencing?

I am using IZON qEV2 columns to isolate exosomes from 100 ml of cancer cell conditioned medium. in NTA analysis I usually see concentration of 2x10^9 particles/ml. But when I proceed with this...

20 February 2021 6,879 3 View

Blast analysis of RC,UHPC slabs using material model *MAT_72R3 in LS-DYNA?

20 February 2021 5,984 3 View

Any suggestions or recommendations on a good publishing house based on the African for academic books in the field of Communication and media studies?

I am currently working on a book on Public Relations (from an African perspective) and the rationale behind the book is the fact that as instructors in this field, we borrow instances and case...

19 February 2021 2,482 2 View

Good day sir/ma. Please, where can I have access to equipment for conducting XRF, XRD and SEM analysis?

I'm using blast furnace slag for stabilizing subgrade soil.

17 February 2021 7,333 3 View