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Questions related to Media
There have been instances when politicians have accused deliberate negative coverage on the part of media. Question is, how true is the same especially in the case of the phenomenon of political...
09 September 2017 2,104 2 View
I am using the Chemicon (now Millipore) Cell Invasion Assay kit (cat. ECM550) to test invasive potential of HTR8 invasive trophoblast cells when our gene of interest is knocked down. I had...
09 September 2017 2,848 1 View
I just isolated novel bacterium in target minimal media. There's no information about the bacterium, I just have 16s rDNA sequence. Is there a way to know if a newly discovered strain is...
09 September 2017 6,232 4 View
i have used MRS, MRS + Cysteine, MRC + BCP, i get gram-positive rods but they turn out to be catalase positive
09 September 2017 9,196 3 View
Need to germinate Brassica napus seeds
09 September 2017 8,110 6 View
I want to quantify proteins in serum free conditioned media. My only issue is that my media has phenol red which reacts weirdly with BCA's working reagent. I suspended my standards (BSA) in...
09 September 2017 1,130 7 View
I have a aqueous solution of an organic compound, initial 400 ppm. later on after treating the solution the organic compound is degrading. let us say, I am finding 200 ppm, 100 ppm, 50 ppm and 0...
09 September 2017 9,104 4 View
I have tried several different extenders (from phisiological saline,PBS, via different types of TRIS to HTF), but for all of them the motility was not good (
09 September 2017 465 3 View
hi, all. I am facing problem in the calculation to prepare minimal media (composition below) that contain 10 ppm chromium. I want to prepare 100 ml minimal media contained 10 ppm of chromium. can...
09 September 2017 4,712 1 View
How to carry peritoneal macrophages in travel?
09 September 2017 1,057 1 View
I can't get these cells to grow! I have tried a couple different CO2 %ages, FBS concentrations, and tips on whether toget rid of or keep floating cells. Currently, I'm at 15%FBS in DMEM and 5% CO2...
09 September 2017 5,112 1 View
Hello everyone, I am working with mycobacterium abscessus for my thesis. I have one question regarding its growth. Because I am always facing some problems with M.abscessus like sometimes the...
09 September 2017 2,248 2 View
I take maybe two weeks try to solve this problem but it's should be better to ask in this community. My work is to do the agar dilution for my organism Neisseria gonorrhoeae using GC agar base +...
09 September 2017 8,520 7 View
We want to look at TGF-b production in out primary cell cultures from mice but traditionally we have used 10% FBS with our media. The problem is that FBS has TGF-b present normally giving us very...
09 September 2017 6,229 3 View
The Republican candidate Donald Trump received such negative coverage during the Presidential Election campaign last year which was unprecedented in the history of the election whereas Hillary...
05 September 2017 6,967 6 View
I inoculated the cultures on PDA and Oat meal agar media, kept for 4 days in dark and shifted to normal light/dark cycles for another 6 days. But, i am not getting spores? Please advice me how to...
16 August 2017 413 0 View
I have in the past collected at the end of 7 days ~50 million macrophages from 1 mouse. I am in recent weeks getting about 10million macrophages from 1 mouse. Please include precise nature of...
08 August 2017 1,967 1 View
I need an explanation and that which bacteriological madia can be used for cultivation of moulds.
08 August 2017 9,974 2 View
I have froze PMBCs in Complete D-MEM. Should I be thawing these samples in the same media as I froze them or switching to RPMI for the thawing process? L-glutamine is present in RPMI but not in...
08 August 2017 672 4 View
if I am, for example, using BG11 media using "https://utex.org/products/bg-11-medium" how could I get a reference for it. I search papers and couldn't find authentic References for it
08 August 2017 9,308 0 View
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08 August 2017 7,888 0 View
How is citric acid beneficial to microalgae growth?
08 August 2017 1,666 4 View
I am currently culturing human astrocytes in full serum but I read it is recommended to starve the cells before treating them. Could anyone tell me how long these cells would last in Astrocyte...
08 August 2017 3,957 3 View
I am checking the mRNA expression of estrogen receptor along with other genes following estrogen treatment in MCF-7 cells. But I am treating cells with 100nM E2. Is it a very high concentration...
08 August 2017 560 2 View