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Hello everyone. i want to isolate and culture bacteria from infected non vital teeth. predominantly the anaerobes are responsible for the primary infection. my question is can i use BHI media for...
10 October 2017 5,480 1 View
I've been trying to detect nitrite from bacterial cells cultures in which the bacteria had a construct expressing bNOS. Tried with a commercial kit (from sigma. This one also converts nitrate to...
10 October 2017 5,550 2 View
10 October 2017 8,335 1 View
Hi every one, I have a problem in isolating bacteria capable of degrading sulfamethoxazole (SMX). My inoculum is granular activated carbon collected from lab biological activated carbon filter....
10 October 2017 752 2 View
How do i measure watchdog role of print media in content analysis of newspapers? If anybody has this type of study paper please share me?
10 October 2017 2,536 5 View
We just started to culture THP-1 cells and treat them with PMA (100nM) for 72 h. Afterwards we change the media daily as recommended. Does anyone have experiences with serum-free culture in the...
10 October 2017 7,315 1 View
I've been seeding some human keratinocytes onto different substrates/conditions which demanded different media, from a stock of cells suspended in Keratinocyte Serum-Free Media (KSFM),...
10 October 2017 5,974 3 View
I have been growing my hybridoma cells inGibco Hybridoma SFM . I have cryopresrved them using 92.5% medium (50:50 ratio of fresh to conditioned media) +7.5% DMSO , the viability was 87%. i froze...
10 October 2017 860 1 View
I have attached an image and graphs of the nitrocellulose membrane from the Spot blot assay. My question is regarding the BM8 lanes. From the immunofluorescence analysis it shows that cells in...
10 October 2017 7,747 2 View
I am looking for a media that gives optimum growth of Cellulomonus and Cellvibrio mixtus. It would be of help if anyone can suggest the proper growth condition of the same.. Thanks!!
10 October 2017 391 3 View
Marketing faces a huge challenge in the digital era. The digital marketing landscape is growing very fast, but the hope that digital marketing would replace traditional marketing has not become a...
25 September 2017 3,111 13 View
I am using Matrigel Corning for cell invasion assay. How can I make a thin Matrigel layer?
21 September 2017 2,261 2 View
Dear all, I am wondering how to evaluate the purity of monocyte derived macrophages (MdM) using flow cytometer. MdM are obtained by adherence isolation, through culture of purified CD14+ monocytes...
09 September 2017 5,491 2 View
The Republican candidate Donald Trump received such negative coverage during the Presidential Election campaign last year which was unprecedented in the history of the election whereas Hillary...
09 September 2017 5,020 6 View
There have been instances when politicians have accused deliberate negative coverage on the part of media. Question is, how true is the same especially in the case of the phenomenon of political...
09 September 2017 2,168 2 View
I am using the Chemicon (now Millipore) Cell Invasion Assay kit (cat. ECM550) to test invasive potential of HTR8 invasive trophoblast cells when our gene of interest is knocked down. I had...
09 September 2017 2,948 1 View
I just isolated novel bacterium in target minimal media. There's no information about the bacterium, I just have 16s rDNA sequence. Is there a way to know if a newly discovered strain is...
09 September 2017 6,283 4 View
i have used MRS, MRS + Cysteine, MRC + BCP, i get gram-positive rods but they turn out to be catalase positive
09 September 2017 9,252 3 View
Need to germinate Brassica napus seeds
09 September 2017 8,154 6 View
I want to quantify proteins in serum free conditioned media. My only issue is that my media has phenol red which reacts weirdly with BCA's working reagent. I suspended my standards (BSA) in...
09 September 2017 1,201 7 View
I have a aqueous solution of an organic compound, initial 400 ppm. later on after treating the solution the organic compound is degrading. let us say, I am finding 200 ppm, 100 ppm, 50 ppm and 0...
09 September 2017 9,197 4 View
I have tried several different extenders (from phisiological saline,PBS, via different types of TRIS to HTF), but for all of them the motility was not good (
09 September 2017 531 3 View
hi, all. I am facing problem in the calculation to prepare minimal media (composition below) that contain 10 ppm chromium. I want to prepare 100 ml minimal media contained 10 ppm of chromium. can...
09 September 2017 4,770 1 View
How to carry peritoneal macrophages in travel?
09 September 2017 1,120 1 View