peptides eluted in 1%formic acid /60 % acetonitrile. i have tried to partially quantify the peptides at 280 nm in a nanodrop. the reading was about 2.3 ug/ul in 6ul but with out any peak. should i still go for the nano lc ?
If you have access to a MALDI-TOF this will be the quickest way to evaluate your sample. In 10 min you'll know if the sample is good for LC-MS/MS.
If not, then the problem is much harder for you. Have you run similar samples and did they also give low values with the nanodrop? Can you load a small amount on a short run to assess sample amount using LC-MS/MS? Are you concerned about time or cost considerations? Is the sample precious, or can you easily prepare more? For any reason do you suspect something has gone wrong with the sample prep? Weigh up what you know and don't know and what is important. You should then be able to make a decision...
Thank you sir. I should do a short run and check. I was just concern about the fine reading in a nano drop with out a peak . this is my first attempt and trying all possible QC before running the sample in lc.
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