Through the web I could find only 6 to 10 stationary phases like C1 to C18 and cyano amino diol etc. I opt to get a detailed list. Please suggest a reference.
What study are you doing with this information? It is very much easier to focus more on Mobile phases since they are easy to manipulate.
Since the basis of chromatographic separation is dependent on polarity, electrical charge and size of the molecule, you may benefit from going though this link for better understanding:
http://www.waters.com/waters/en_MY/HPLC-Separation-Modes/nav.htm?cid=10049076&locale=en_MY
As our friends mentioned above the best place is hand book of chromatography, or better hand book of stationary phase. Obviously you are trying to separate the components of a sample, and you have chosen a correct method, because the polarity of the analyte must be matched with the stationary phase, if not the retention times would be too short.
As you will have seen from the earlier cited link to Waters, they are quite good at providing this kind of information for their products. Here is another link that compares their columns by polarity etc (mainly reverse-phase):
http://www.waters.com/waters/promotionDetail.htm?id=10048475&locale=en_GB
I have found that it can be useful to work from an appropriate column suggested by this this type of guide, and then look in detail at its properties, in order to find out which columns from other suppliers match most closely, if you do not wish to be restricted to Waters products.
1 / 0 · 7 hours ago
Sorry, I have re-posted this as I had to delete an accidentally duplicated RG account, so my answer appeared with a 'deleted' profile
That is a fairly general question. You may have Adsorption chromatography, where the stationary phase is a polar adsorbent (e.g. silica) and the mobile phase is nonpolar (e.g. hexane). Then you may have reverse phase, where the stationary phase is nonpolar (e.g. the C18 you mention) and the mobile phase is polar (e.g H2O/Acn). Go further and you end up in ion exchange chromatography. For size exclusion or affinity chromatography, polarity is not the driving force for separation.
For further reading I suggest a general textbook on HPLC such as Katz et al (1998) Handbook of HPLC or Lough and Wainers 1995 High Performance
Liquid Chromatography. Fundamental Principles and
Practice.
Hi Tushar: May I ask why do you ask this question? It would help us to know why because your question is very general and a search on the web, at column vendor sites, would rapidly provide the data you need.
There are about one thousand general purpose HPLC columns on the market today sold under a number of names and types. Out of that group there are perhaps 15-20 basic categories of columns (which have significant differences in properties). There are subsets of these column types which would expand on the number too. It all depends on what you are trying to find out.
- Badges
- Science method