How can I avoid degradation of compound by water during RP-HPLC analysis?

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Is There Any Feasible Method To Test Fluorescent Compounds Other Than Fluorescence Spectrometers ??

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Mike Robinson

Protecting groups are common means, but you need to share your cpd for specific answers/suggestions.

Kashyap Thumar

you should refer following article. it may be helpful to you.

M. Isabel Pinto

At what temperature are you doing the analysis?

Mathias Christian Franch Andersen

25oC. Higher temperatures results in increased degradation. Lower temperature is not possible since our collaborators does not have possibility to cool their systems.

Which eluents? Maybe change the eluents.

Paul Anton Steenkamp

The conditions you listed that would minimize the degradation of your compound, suggest the use of HILIC conditions, eg. low water, high organic at a pH of 6 - 8. You mentioned that you tested HILIC - how did the compound react under these conditions?