3T3-L1 cells, is this a bacterial contamination or a cross contamination with different cell line? or something else?

Hi, I am experiencing some difficulties with 3T3-L1 cells culturing recently. I am working with 3T3-L1 cells for almost 4 years now and have seen bacterial contaminations/ unhealthy cells and...

13 February 2021 4,040 3 View

What solvent should I use to dissolve Mosapride citrate for in vivo studies?

Hello, Can you please give your suggestions to dissolve Mosapride citrate (a gastric emptying agent) for oral gavaging in mice? This chemical dissolves in DMSO. However, using a high...

13 July 2020 4,494 3 View

Can someone please help me with the dose calculation in animals?

Hello, I wanted to know the method of calculating the converting the concentration of a sample in gram/100 gram diet to oral administration concentration in mg/kg body weight of mice. For example,...

08 January 2020 8,418 2 View

Can I use Tris- hydrochloride instead of TRIS?

Hi, I am preparing STE buffer for genotyping in the method following. I want the buffer to have a final pH of 8. Tris - 100mM EDTA- 5mM SDS-0.2% NaCl- 200mM Is TRIZMA base is more suitable or...

23 January 2019 4,989 2 View

Is anyone familiar with the SE520EXi flow cytometry machine (produced by StratedigmLtd)?

We want to buy a new flow cytometry device for our laboratory and because this instrument is relatively new, I would appreciate if someone could give me a feedback on this specimen (advantages and...

07 August 2014 4,755 0 View

For ZnO temperature sensor what type of bimetallic strip can be used in the dip coating process?

I'm currently doing a temperature sensor using nano particles so in the process we use a bimetallic strip on which the nano rods are grown so i want to know what type of bimetallic strip can be used??

01 January 1970 6,740 2 View

Is There Any Feasible Method To Test Fluorescent Compounds Other Than Fluorescence Spectrometers ??

Is There Any Feasible Method To Test The Efficiency Of Fluorescent Compounds Other Than UV Spectrometers ? Suggestions Would Be Appreciated !

02 March 2021 5,785 3 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

Samples to study circular dichroism (chirality)?

I want to build a low-cost polarimeter to study circular dichroism (chirality). What type of samples can I use? I am looking for samples that can be found outside the laboratory.

01 March 2021 9,101 3 View

How to accurately quantify chitin?

Hi all, I have been working on the quantification of chitin in insect samples and feed for a while but I can notice that the quantification methods (e.g. exposure to HCl, NaOH, and weighing sample...

01 March 2021 1,843 2 View

Unknown debris sheets observed in cell culture flasks?

Hi, We culture neural progenitor cells in T175 culture flasks coated with PLO-Laminin. We have successfully grown these cells with our protocol for months. Recently, we observed large sheets...

25 February 2021 3,443 4 View

Can anyone help with Schistosoma identification?

Can anyone identify what kind of Schistosome species this might be? Morphological features of cercariae and adults resemble Trichobilharzia, though I am fairly new to the field of parasitology...

25 February 2021 7,760 6 View

Can we recognize signs of the "volatility clustering" property of a time series in its time domain plot?

My purpose is to recognize the volatility clustering property without statistical tests such as The Lagrange Multiplier (LM) test.

24 February 2021 4,150 3 View

Suppose we have a banana seller cart consisting of 4 wheels, weighing about 300 kg. How much torque motors are needed to pull of push it electrically?

I am thinking of electrifying the street food carts while harvesting energy using solar panels on top.

22 February 2021 4,323 3 View

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22 February 2021 9,932 11 View