How do I prepare benzonase buffer?

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Jukka Westermarck

Benzonase buffer:

50mM Tris-HCl

2mM MgCl2

K M Taufiqur Rahman

Thanks Jukka. Is there any reference that I can use for this purpose. Could you please tell me about the exact measurement of Tris-Base which can easily make the pH=8 after mixing with 50mM Tris-HCl.

Please e-mail the Xi Qiao who did the experiment: [email protected]

Hello,

Thanks for your information.

Waheed Ur Rahman

Dear KM Taufiqur Rahman or Jukka Westermarck, could you tell me the pH of the Tris-HCl in this buffer ?

or if you have detailed recepie, I would be happy to get it from you. thanks