I am hoping to perform in either fresh frozen or fixed mouse brain tissue. Thank you!
Hallo Mohammad,
put formalin fixed floating brain sections after a short rinse in destilled water in 50% ethanol for 1-2 min. Then transfer the sections in a saturated Sudan IV in 50%ethanol/ aceton à/à (equal parts) for 5 -10 min at 37°C or room temperature. Then rinse the sectoins carefully in 50% ethanol and destilled water and mount them on superfrost plus slides . If you like you can counterstain with haematoxyline. Mount your sections after staining in a water soluble mounting medium.
Hi Mohammad,
remembering the synomyms of SUDAN IV, namely:
- Biebrich scarlet R fat soluble,
- Fat Ponceau R or 4,
- Lipid Crimson,
- Oil Red IV,
- Scarlet Red Scharlach,
- Solvent Red 24, and last but not least:
- Sudan R or BB
while staying at the University of California at San Diego, California it should be possible for you to "ask" either "Google" inserting:
| Sudan IV lysochrome staining protocol |
(yielding approxim. 1,780 results): from the first 10 results only as examples:
@https://www.sigmaaldrich.com/content/dam/sigma-aldrich/docs/Sigma/Product_Information_Sheet/1/s4261pis.pdf -
@http://www.newcomersupply.com/documents/staining/procedures/Sudan%20IV.1400.pdf -
@https://www.cvrc.virginia.edu/Owens/files/Staining_protocols/Sudan_IV_Stain.pdf -
@http://stainsfile.info/StainsFile/dyes/26105.htm,
If searching in / with Google for:
| Sudan IV lysochrome brain sections staining protocol |,
you end up with 946 results. Searching for
| "Sudan IV" "lysochrome" brain sections staining protocol |
yields 498 results, the first 10 or 20 will serve your task and problem solving...
You also can try and narrow your task searching in Google scholar (e.g. using the same search keywords/phrases as mentioned above: e.g. | "Sudan IV" "lysochrome" brain sections staining protocol ) | at https://scholar.google.com/
approx. 28 results, interestingly, the article found on
https://intarchmed.biomedcentral.com/articles/10.1186/1755-7682-4-5 for their SUDAN IV-Method the authors refer to REFAT et al, 2008: "The Induction of Cytochrome P450 1A1 by Sudan Dyes, J BIOCHEM MOLECULAR TOXICOLOGY 22,No 2", which unfortunately is available only as PPV ( Pay Per View) : http://onlinelibrary.wiley.com/doi/10.1002/jbt.20220/abstract;jsessionid=13D8AC33067F3CAB0B903E4B3F5CB2FF.f04t03. So their method unnecessarily is obscured a reason of which I can't understand.
If you search NIH-PubMED for | "Sudan IV" lipid stain | (cf: https://www.ncbi.nlm.nih.gov/pubmed/?term=%22Sudan+IV%22+lipid+stain ) you'll find at least 20 papers dealing with Sudan IV
So in the end, if you can't find any solution out of the many literature references, articles and company pamphlets found by the former literature searches, you have - as the first or the last choice the method Ute Neubacher acknowledgedly has provided (if you get positive / good-excellent results please don't forget to cite Prof. Dr. Ute Neubacher with this RG-thread as the secret source of your success!)
- Badges
- Science topic
- Similar topics
- Methodology
- Laboratory
- Histological Techniques