Are there any candidates for an Einstein's bare cosmological constant?

It is commonly accepted that General Relativity has its own 'bare' cosmological constant that contributes together with the cosmological constant resulting from vacuum energy density for an...

23 February 2021 5,448 8 View

What is the best method (software) to count pixels in a digital image?

I'd like to carry on an accurate count of pixels in several images in order to compare them. There is a way by using Photoshop, but... does anyone know any other better method or software for...

05 November 2020 3,536 3 View

Combining multiple spring constant in umbrella sampling?

I have experienced insufficient sampling for high energy states in particular windows. The spring constant is not enough stiff to keep the value of the reaction coordinate. I know that you combine...

23 September 2020 7,800 2 View

What do you include in writing an abstract of a social science article?

Some have 3 elements- rationale/background, methodology, and results; while others have four or five that include objectives/questions, conclusion with implications.

19 August 2020 8,203 25 View

What is the simplest method of developing a theory? The GT approach allows theorists to develop a theory in an uncertain time?

It seems that the authors of some books present grounded theory (GT) as the only approach or method of developing a theory. By the name of the approach, it may confuse beginner researchers.

12 August 2020 5,734 12 View

What are the benefits of writing a systematic literature review?

SLR- Systematic Literature Review. In this, you don't need to go to the field to collect the data. You just need a desk. When is SLR necessary and is not necessary?

12 August 2020 6,009 18 View

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05 August 2020 9,805 2 View

COVID-19 contamination through the airborne transmission mode?

Does anybody know about reported evidences of COVID-19 contamination through the airborne transmission mode, besides the two papers about the Guangzhou restaurant? It is important that the...

31 May 2020 4,881 4 View

What is the path loss formula for a drone-to-ground communication?

How can I estimate the propagation loss between an unmanned aerial vehicle (UAV) or drone, and user equipment (UE) on the ground? is it safe to assume a line-of-sight scenario? how about the...

24 May 2020 3,517 5 View

How to start a bioinformatic analysis of bisulfite sequencing data?

I'm just learning how to perform an analysis of methylation patterns using bisulfite sequencing data, but I have some questions: 1. What database I can use for obtain bisulfite sequencing...

10 May 2020 1,116 1 View

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02 March 2021 3,060 3 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

Interested to stain mice tissue with WGA 488 conjugate, anybody has a protocol to recommend?

Interested to stain mice brain tissue with WGA 488 conjugate, appreciate if anyone willing to offer me some guidance or suggestions! Thank you!

28 February 2021 3,951 2 View

What could be some reasons why unstimulated monocytes are getting activated?

Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.

28 February 2021 7,883 3 View