If possible can i get online version of odd one out task for kids?

Does anyone know from where can i have the online version of odd one out task as a measure of non-verbal working memory for kids (4-7)yrs to assist my research?.. Thank you

18 June 2019 5,913 1 View

How to find and differentiate the cache access time and Ram access time of a program?

25 April 2017 5,314 3 View

Dynamic cache partition of the cache through Xen scheduler?

20 February 2017 6,987 1 View

What should be the minimum percent of identity and coverage of blast hits for considering as gene sequence?

Hello group, I had predicted peptide sequences from denovo assembled contigs using abinitio (GENSCAN) approach and subjected it to similarity (BLASTP) search to identify genes in the assemble...

03 November 2015 5,833 2 View

What are the reasons for PCR failure when DNA is extracted from degraded feces, conserved in 70% alcohol?

31 October 2014 1,823 16 View

How many intrusion responses have been developed in cloud and mobile cloud technology in 2013 and 2014?

How many intrusion response systems have been developed up till now?

03 April 2014 2,237 2 View

What is the best method to extract dna from faecal matter?

Using the method using ezyna cycle pure kit, omega.

19 November 2013 356 7 View

How is the microsatellite characterization of DNA helpful for conservation of species?

Any comments?

06 November 2013 1,127 4 View

Has anyone had luck using cell lines other than HEK 293 (such as COS1) to package lentivirus?

I am looking to package lentivirus in endothelial cells but I am opposed to using 293 cells due to personal ethics regarding the source. I have done some reading and saw that COS1, COS7, & CHO...

25 February 2021 3,030 1 View

Wat is the half-life of luciferase protein in mouse tissue when delivered using mRNA luciferase?

Hello, I I am validating a system to deliver mRNA using nanoparticles to a mouse model. I am initially using mRNA for firefly luciferase to be able to do live imaging in the mouse. After that, I...

17 February 2021 2,536 3 View

Is there a way in which microglia can be extracted from mice without killing them ?

I'm aiming to use an adoptive cell transfer approach, with microglia in mice with ALS. So far all the protocols I have seen, require sacrificing the mouse. I'd like to re-infuse the microglia back...

11 February 2021 9,509 1 View

HiPSC positive for GLAST in FACS - why?

Hey, I'm working on hiPSC differentiation into astrocytes. I stained my cells (hiPSC stage) with a GLAST (ASCA1) antibody for FACS and the cells showed a strong positive signal. As far as I know,...

09 February 2021 3,383 4 View

What is best technique to quantify necrotic cell death of specific cell populations?

I currently using FACS (Aurora Cytek) to quantify necrotic Gr1+ cell death from the spleens of immune competent mice. I target Gr1+ cells in the spleens of tumor-bearing mice using a photodynamic...

08 February 2021 733 6 View

What recipe of homemade lenti-virus concentrator do you use?

My lab is using a lenti-X concentrator. And we are looking for a homemade recipe. I have tried this recipe....

08 February 2021 5,965 1 View

SARS-CoV-2 Spike Protein Western Bands?

Hello, I am western blotting SARS-CoV-2 spike protein from lentiviral using an antibody against S1. To prepare samples, I mixed concentrated lentivirus 1:1 with 2X Laemmli buffer and boiled...

07 February 2021 3,643 4 View

Choise of cell line for neurobiology?

I am working with myelin-related proteins and interested to do cell experiments with my target protein in cells. I have created lentiviruses for my proteins, and mutants and also CRISPR set-up for...

02 February 2021 8,083 1 View

Why is my GOI not expressing after lentiviral transduction despite RNA increase and protein is detected in transfection?

Hi, I'm trying to create a stable overexpression line with EF1a-FlagGOI-IRES-GFP using lentivirus packaged with pxPAX2 and pM2G. After transduction of 293T with 8ug/ml polybrene, I was able to...

29 January 2021 1,878 4 View

Does anyone know why I would have unexplained emission overlap for flow cytometry?

Hello, I am new to flow cytometry, and was just using a sample cell population to train with the technician. The cells were PAC-1 cells split into four groups. Unstained, transiently transfected...

28 January 2021 969 2 View