human Topoisomerase is a HOMODIMER Protein (AA). Now i want to mutate one chain to produce a heterodimer version (AA*). but there are chances of production of three products (AA, AA*, A*A*). So the question is that how i should place the two Genese wild type (A) and mutant (A*) in E coli expression system that only one required form AA* is produced?
Michael J. Benedik
So you can easily express the two different proteins in the same cell, either by using 2 plasmids, or by by using 2 expression cassettes on the same plasmid, or by creating an operon with both copies of the gene.
HOWEVER you can not make it so that only the AA* is made, you are going to get a mixture of AA, AA* and A*A*.
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