How could gene editing be used to extend human lifespan?

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My cfDNA extraction protocol using silica beads works with plasma collected in COD SAGM bags but not with plasma collected in K2 EDTA tubes. Why so?

I am currently performing cell-free DNA (cfDNA) extraction from plasma using an in-house developed kit that employs silica-coated magnetic beads. My protocol works effectively with plasma obtained...

28 July 2024 3,559 0 View

Sample preparation for characterizing hydroxyapatite nanoparticles?

Hello all! I have synthesized hydroxyapatite nanoparticles and have to characterize them with DLS and SEM analyses. Can someone please let me know the sample preparation methodology for DLS and...

06 April 2024 7,614 5 View

Publication papers for speech recognition?

I need to require recent publication papers for speech recognition

01 April 2024 4,103 3 View

Which is the more accurate method to calculate LUMO value for a D-A system?

Which is the more accurate method to calculate LUMO value for a D-A system? Either it from absorption edge or from CV measurement on reduction side?

15 February 2024 5,635 1 View

Why are my cell-free DNA elutes translucent after cfDNA extraction using plasma samples?

I am carrying out cell-free DNA extraction using an in-house buffer system and magnetic nanoparticles. My qubit readings and tape station data look good. But upon using some plasma samples (2 out...

11 January 2024 9,426 0 View

Hello, Could someone please share with me an equation to calculate the electric field strength of an RF power supply of 250 W?

This is for my postgraduate research work.

22 December 2023 5,138 2 View

Im not able to see any protein bands.This is how our Nylon membrane looks after taking out it from semidryer.whats the problem?

we are applying 24v for 40mins.After that our nc mebrane is becoming like the image i posted, we tried to change current and timing, every time it is becoming like this.

29 October 2023 9,589 0 View

Is it possible to find the crystallite size of thin films from XRD?

It is known that W-H plot can be used to find crystallite size of nanoparticles. But, is it correct to follow the same method to find crystallite size of thin films?

10 October 2023 8,696 6 View

How to Determine the LOD/analytical sensitivity of a molecular assay for bacterial detection in CFU/ml?

Hi all, I need support in calculating the analytical sensitivity of molecular assay ( detection of DNA) for detection of an bacterial infection from oral swab samples. Usually we spike various...

11 August 2023 1,460 2 View

We are facing the problem with agarose gel electrophoresis.after completing running the gel half of the agarose is not stained?

Even though we are mixing the ethidium bromide properly before agarose gel casting We are facing this problem of uneven staining.Its not that the staining is random, but exactly half.We tried...

11 May 2023 409 3 View

Why Do TDS and EC Increase with Larger Wastewater Volumes, While BOD and COD Decrease?

I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...

09 August 2024 9,621 0 View

Could it be a cell culture contamination?

Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...

09 August 2024 2,824 3 View

Is there any way to quantify bacterial and fungal cells in their mixed culture?

I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...

07 August 2024 7,535 4 View

How to normalize and take the significance of the MTT OD values with 3 replicates for the same cell-line?

Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...

07 August 2024 8,106 4 View

Why activated CAR-Jurkat cell could not kill targets?

Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...

06 August 2024 641 2 View

Weak DAPI staining after immunohistochemistry - how to improve?

After immunohistochemistry of previously fixed in PFA and EtOH and then frozen 20 μm sections of zebrafish brain, DAPI staining is very weak (right) compared to the same sections stained without...

05 August 2024 9,637 2 View

How to isolate lymphocytes from mouse spleen?

I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...

04 August 2024 9,913 7 View

How to Add Missing Water Molecules in Protein-Membrane Simulations?

I have protein-membrane simulations (PDB, PSF, DCD) and have noticed that water molecules near the protein are not visible in the simulations. How can I fix this issue? Is there a way to place the...

04 August 2024 1,200 2 View

Does long-term culture of murine Monocytes result in macrophage differentiation?

Hi, I am isolating monocytes from the bone marrow using the Mouse Monocyte EasySep kit. I want to treat these cells and monitor expression of specific markers over the course of 10 days. I will...

04 August 2024 7,282 2 View

To perform transfection with DharmaFECT Duo in AGS cells. Could you tell me what the ideal concentration is to avoid significant cytotoxicity?

I would like to perform transfection with the reagent DharmaFECT Duo (Horizon) on the AGS cell line. Could you please inform me of the optimal concentration to use without causing cytotoxicity in...

03 August 2024 3,851 1 View

Gregory Dressler

The problem with anti-aging methods is any clinical trial would take so long to achieve significance that no one wold invest in it. Measuring longevity takes a long time by definition!

Dasari Keerthana

Gene editing could extend human lifespan by:

1. **Telomere Extension:** Lengthening telomeres to slow aging.

2. **DNA Repair Enhancement:** Improving genes responsible for repairing DNA damage.

3. **Senescence Prevention:** Removing or altering genes that cause cells to age and stop dividing.

4. **Mitochondrial Health:** Editing mitochondrial DNA to prevent age-related energy decline.