I am continuously getting some precipitates in pockets in gel electrophoresis. I have tried with incubating the samples at around 40°C for 1hr and also I have tried with low concentration of agar. I am still getting this problem.
what samples are you running in what amounts and dissolved in what? What type and concentration of gel and gel running buffer are you using?
Can you post a picture please?
If you are running DNA then purify it, there may be higher amount of protein present in your sample.
Hello Narayan
1st you must mention here what sample are you running.Sample amount and storage conditions.
2nd check the gel preparation method
3rd again make sure your sample preparation method.
I want to know the effect of KCl fertilizer application on chilli and tomato farm.
20 June 2024 3,486 2 View
I randomly interviewed 250 poor people and 250 non-poor people. Considering 1 for poor and 0 otherwise, does estimating a logit model aiming to capture the probability of becoming poor make sense?...
02 June 2024 2,326 2 View
I found atomic% and weight % of doping element decreased in the resulting compound on increasing its concentration.
28 May 2024 2,455 2 View
I have conducted a mushroom cultivation trial for study purposes. I inoculated my substrate with oyster mushroom spawn about a month ago. Now, it has completed the incubation period. There is a...
21 January 2024 5,359 4 View
Along with scientific studies, your scientific thoughts are highly welcomed.
03 November 2023 9,570 2 View
I want to measure the work function of fused silica. Is it possible to measure the work function using UPS? I have seen several papers using UPS, but did not get the concept behind that. Does...
02 November 2023 3,779 4 View
Most of paper refer this model to induce NAFLD however, why most of method followed DMEM without FBS to initiate NAFLD
01 November 2023 3,510 0 View
I need to make an input file for quantum espresso.
16 October 2023 1,232 3 View
as i have joined a progressive school in teaching practices.
19 August 2023 4,973 7 View
What is the equivalent energy of the element Sulphur? with appropriate reference. I need to calculate the energy input from Sulphur fertilizers. Thanks in advance.
05 August 2023 4,731 2 View
I have been working on Red blood cell-derived extracellular vesicles as Antisense Oligonucleotide (ASO) carriers. We normally run agarose gel to quantify the loading efficiency. I used naked ASO...
06 August 2024 3,129 2 View
It's an end-point PCR protocol. I'm using 1.5% agarose gel with SyBR Safe dye and TBE as a running buffer, visualization on BioRad XR+ system. I was primarily thinking of primer efficiency,...
01 August 2024 4,672 4 View
Some Staphylococcus aureus strains Inhibit the growth of Mycobacteria in Mueller Hinton Agar medium containing 10% OADC. Do some Staphylococcus aureus strains have in vitro antimycobacterial activity?
29 July 2024 10,022 2 View
I am cloning an overexpression plasmid with my protein of interest tagged with mScarlet. After transforming my ligated product into DH5α bacteria and plating on LB agar, I noticed colonies with a...
22 July 2024 5,952 6 View
Greetings. I’m currently running a nested pcr for giardia. My mastermix comprised of 3mM Mgcl2, 5unit of taq polymerase, 0.2uM of forward and reverse primers each respectively and 0.2mM of dNtp...
22 July 2024 9,760 5 View
I’m having difficulty achieving high RNA integrity in my samples. Although the 260/280 and 260/230 ratios are satisfactory after RNA extraction, the RNA samples show signs of degradation when...
22 July 2024 155 4 View
Sometimes I see the shadow like bands and its not true band. I want to know that what's the reason for it. I am using 2% gel for running genotyping samples I have uploaded the gel picture in both...
19 July 2024 148 6 View
I have been running native page for FAM DNA substrate ( fluorescence samples) for protein DNA binding reaction. Binding is there but towards the end of the lane , I am loosing signals...
17 July 2024 6,212 4 View
I start with IDT ordered 124 bp ssDNA that is PCR amplified, then I use a QIAgen agarose gel extraction to get my exact product, and I'm trying to purify ~95bp RNA by denaturing PAGE to prevent...
16 July 2024 4,604 0 View
I have conducted molecular marker amplification experiment by PCR and performed PAGE with silver staining. Now i have to score all my gels for further genetic analysis. Manual gel reading is...
12 July 2024 7,145 2 View