How to get rid of precipitates in wells in gel electrophoresis?

More Narayan Parajuli's questions See All

Is folic acid cytotoxic to human kidney cells ?

My group is interested in folic acid cytotoxicity in human kidney cells. Are there any reports available showing toxic effects of FOLIC ACID as in the case of mice kidneys? Any insights will be...

19 October 2020 2,273 3 View

How to generate a Force which has a duration of 0.3 ms and allows excitation of waves of frequencies up to 80 kHz in matlab.?

Actually this is the impulse force, which is to be applied on the rod element?

09 September 2020 8,080 3 View

How to calculate Abaqus software compatible material properties from the data described below?

From testing data: yield stress, ultimate stress, total strain, ratio of strain at elastic limit by strain at strain hardening and the ratio of elastic yield modulus to the strain hardening...

06 September 2020 5,279 10 View

How to find the flame speed from fluent??

I had the simulation upon combustion in ANSYS FLUENT. Reaction was based on the "Federick and Dryer" global mechanism. Solution was able to obtain convergence but i need to find the flame...

18 June 2020 1,614 6 View

What is a popular book for understanding laser fundamentals and evolution and types of lasers?

I looking for books, channels, videos and PDFs regarding laser and particle interaction and evolution of laser power and laser types and how laser makes the physical changes on the ceramic...

05 April 2020 3,396 25 View

How to determine the number of clusters in Cluster Analysis using SPSS?

I am trying to analyse students data to determine the common characteristics of mathematics students. Cluster Analysis would be valuable to get these common characteristics. Any idea or any...

27 March 2020 2,949 5 View

I would like to know if there is anyone who is working on CEP126 gene(also known as KIAA1377)??

As there is very little data available for CEP126 gene on databases, i would like to know if there is anybody or any lab working on this gene.

31 January 2020 1,860 1 View

How Can I get the datasets for in-vehicle network CAN bus security research ?

I would like to study the security in in-vehicle network CAN bus. Denial of service (DoS) and Replay attacks would be launched and ways to solve these problems is to be studied. Machine learning...

26 January 2020 3,061 3 View

Calibrating stereoscopic system with a laser line projector and camera?

I want to set up a framework for combining a laser line projector and a camera to extract the 3D coordinates of a target object. I cannot find much literature regarding this using active light...

16 October 2019 3,582 1 View

Can anyone explain the unexpected protein concentration loss in buffer exchange?

I have a stx2 236 TALE-A protein in glycerol stock at -20C. It's concentration is 3.2uM. I did buffer exchange with HEPES buffer 3 times (100uL protein+ 300uL of buffer each time) in a micro...

05 September 2019 4,847 3 View

Protein-aptamer gel electrophoresis help, please??

Hi, I have problems with running gel electrophoresis. I have tried agarose gel electrophoresis and native PAGE. I have two proteins, which have molecular weights of ~30kDa and ~180kDa and two...

03 March 2021 4,275 4 View

Gel electrophoresis result for total RNA samples, 1.5 Agarose gel was used, How to improve the integrity of RNA?

Gel electrophoresis, RNA degradation, RNA extraction from fresh tissue

02 March 2021 5,433 5 View

How to regulate SSCP temperature in simple PAGE?

I'd like to perform single-strand conformation polymorphism (SSCP) in my thesis, however I cannot control the temperature of the vertical PAGE since we are using the conventional tanks. Is there a...

02 March 2021 9,157 1 View

How to grow E.coli on the low salt LB agar plate with 50 or 100ug/ml of Blasticidin?

Hello, I would like to ask how to grow E.coli which resists 50 or 100ug/ml of Blasticidin. I have tried many methods by using low salt Luria-Bertain(LB) agar plate. However, no colonies grow. Let...

01 March 2021 2,027 2 View

Why are my bands weird in this Agarose gel?

Can someone please give me some possible things that could go wrong? Here is my recipe: 0.5g Agarose 50 mL of TAE 1x 1 uL ethyl bromide. Gel was run at 100V for 1 hour. The buffer used is also TAE.

01 March 2021 9,952 3 View

Precipitate after adding laemmli buffer to protein solution containing Potassium Acetate?

Hi, I am running a size exclusion chromatography experiment with a buffer containing Potassium Acetate as a salt. I analyse these fractions through SDS-PAGE. After boiling my SEC fractions in...

01 March 2021 2,622 3 View

Can bacterial suspension be pipetted directly onto petri dish and agar poured onto it?

Hi, recently I encountered a protocol for plating which was not the usual method I have been practicing (pour LB agar onto plate, allow it to solidify, pipette bacterial suspension onto plate,...

28 February 2021 7,292 3 View

Why my agarose gel staining sometimes looks not homogenic but like gradient (see the figure)?

I am worried about this overexposure of the upper part of the gel in the picture. Is it possible that this is the result of too much concentration of ethidium bromide? Why is there such a big...

25 February 2021 8,140 3 View

Difficulty in cloning of 1kb gene into vector?

I am facing difficulties in cloning a 1kb gene into a vector (pJIT163). I have my gene on interest (GOI) in pUC57 and want to clone in pJIT163 using SalI and BamHI restriction sites. I am getting...

25 February 2021 3,221 7 View

My silica gel column dried can I somehoe save the trapped matter?

I tried to separate my substance from a protein in a sillica gel column. By mistake, it dried and cracked. Can I save the trapped substance? Can I repack it ,or at least elute all of the trapped...

22 February 2021 1,789 3 View

Paul Rutland

what samples are you running in what amounts and dissolved in what? What type and concentration of gel and gel running buffer are you using?

Can you post a picture please?

Faryal Ahmed

Hello Narayan

1st you must mention here what sample are you running.Sample amount and storage conditions.

2nd check the gel preparation method

3rd again make sure your sample preparation method.