Dear Yash

You need to search NCBI database for SSRs/ EST-SSRs in desired species. Then either you design your primer or look for previous studies with characterized SSRs of your interest. Once you have sequences of Farward/Reverese primers you can place order to commercial biotech companies for primers pair. Yeah as Poly acrylamide gel (PAGE) has higher resolution power, it will resolve your PCR product more precisely, with even few bp differences. Best of luck

Dear Haris,

I have already design SSR primers and i have pcr products. I am looking for DNA maker ( Ladder ) to run with PCR product on PAGE.

Thanks.

Regards

Yash

Dear Yash

i suggest you to use pUc19 / Mspi DNA Ladder

size range form 34 to 501

and follow my page

https://www.researchgate.net/profile/M_El-Sayed2/contributions