Fluorescence

The property of emitting radiation while being irradiated. The radiation emitted is usually of longer wavelength than that incident or absorbed,... | Contact experts in Fluorescence to get answers

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Questions related to Fluorescence

How estimate protein concentration by alternative method?

Hello to everyone. My work focus on studying PTMs and I have successfully phosphorylate my protein in vitro (Checking the reaction products by SDS-Page, all of my protein should be...

30 March 2023 1,614 3 View

How I can perform the best experiment using dual luciferase assays for gene expression, where to find normalization & fold change calculations guide?

Hello there! Could anyone help me out?? I'm new to Luciferase assays; I would like to develop a dual luciferase assay using a d-Luciferin substrate luciferase as my reporter luciferase and a...

29 March 2023 5,474 0 View

How can I measure the thickness and refractive index of a polymer film deposited on a glass substrate?

What is the easiest way to measure the thickness and refractive index of a film? Note that the available devices are spectrophotometers, Michelson interferometers, and Abbe refractometers.

28 March 2023 3,159 3 View

How to convert concentration in microgram or microgram per ml?

I have submitted my manuscript on mechanistic interaction of proteins with nanoparticles. As i consider nanoparticle as a single molecule and calculated its molecular weight accordingly and...

25 March 2023 574 1 View

I would like to know about the inernal primer in sequencing?

When I did my plasmid in sequencing center, I choose the universal primer for the sequecing. However, I would like to know about the ineranl primers and when we need internal primer for sequecning...

23 March 2023 3,690 1 View

Why do my ligations keep failing?

I am trying to insert four different sequences, indivually, in a backbone. So, I will have four different clones all with the same backbone. I am using a modified pcDNA3.1(+) plasmid that has had...

23 March 2023 3,462 2 View

How to find yellow flags matched for JCR Journals?

In recent days, Clarivate removed IJERPH from JCR and WoS but prior to this move they matched so-called ‚yellow flag’ which means that a particular journal is monitored. How to find current...

22 March 2023 2,782 0 View

Is DIG based in situ labeling better than biotin based?

I found that almost every paper that shows in situ hybridization, uses DIG-UTPs for labeling with fluorescence anti-DIG antibodies. Is there any advantage over Biotin-UTPs and fluorescence avidin...

22 March 2023 2,390 0 View

Why do I get low cell counts for HeLa at high confluency?

Hi everyone. I have started working with HeLa cells recently. I used stocks from someone else who has left the lab and no longer available for answering questions. Anyways, I harvest when my HeLa...

21 March 2023 5,199 7 View

Can T cells de-differentiiate?

Hi, Does anybody know whether effector or memory T cells can de-differentiate into naive T cells, rather than just become exhausted or undergo apoptosis? Thanks

20 March 2023 4,036 2 View

Hi, does anyone have experiences with the transfection of NK-92 cells?

Hi, everyone. It is said that NK-92 is very difficult to transfduce, so how can it be successfully transfected?

20 March 2023 5,145 0 View

Can someone suggest me a good book to read EPR/ESR spectroscopy from a physics POV?

I will be starting working in ODMR (Optically detectable magnetic resonance) spectroscopy , i need a good book which will help me understanding ESR and EPR spectroscopies.

18 March 2023 5,271 3 View

What can i do for finding MOI of phages which can not lyse bacteriophage in liquid phase? It formed a lot of plaques on double layer agar!?

I am working on bacteriophages. In my research, I am looking for a suitable lytic bacteriophage for Staphylococcus aureus. I have isolated a bacteriophage and it has formed a lot of clear plaque...

16 March 2023 767 2 View

The absolute full-range output voltage of the piezoresistive pressure sensor increase with the increase of temperature ?

Dear all: I am studing the pressure sensor, and want the absolute full-range output voltage of the piezoresistive pressure sensor increase with the increase of temperature under the constant...

14 March 2023 8,723 1 View

Using transwell , what should be the protocol for studying migration in T-47D cell line?

Want to do migration assay in T047 D cell line , please suggest protocols

13 March 2023 2,958 3 View

My HEP-G2 cells aren't growing, what can I do?

Hi everyone, I have HEP-G2 in culture with DMEM (4.5 g/l Glucose, +Glutamine, -Pyruvate). I exchanged the media every 2-3 d. Unfortunately, the cells are not growing as intended and I couldn't...

07 March 2023 8,337 3 View

Why fluorescence based metal ion detection is highly sensitive? and selective ?

fluorescence based metal ion detection in aqueous samples is highly sensitive and selective i need scientific reasons for these high selectivity and sensitivity ?

01 March 2023 452 1 View

Why is Cd-hit-est giving output with -/100% identity?

I have a set of 2000 sequences. In that set there are two sequences >Seq1 AAAAAAAAAAAAAAAAAAAAAAA >Seq2 UUUUUUUUUUUUUUUUUUUUU When clustering with Cd-hit-est with default options, these two...

28 February 2023 723 1 View

I ran the colony PCR today..I have attached the image too...Can anyone please help me.out regarding this that why iam getting this genomic DNA?

I ran the colony PCR using bacterial colony of Salmonella Typhimurium using specific primer but I don't get any specific band.So please help me out.

24 February 2023 9,708 0 View

Stem cell line don't differentiate into specialized cells, why this is happen any specific reason behind it?

Cells in a stem cell line keep growing but don't differentiate into specialized cells, why this is happen any specific reason behind it?

23 February 2023 239 2 View

What are the biochemical tests required for identification of Lactobacillus spp.?

When isolating Lactobacillus spp. like for example on MRS media, and you want to identify that this indeed is a Lactobacillus spp., what the are the biochemical tests required to do so? Thanks in...

23 February 2023 3,223 0 View

How to measure glutamate neurons activation?

Good morning all, I have a cell culture, where there are different types of neurons. Then I want to test an effector in this culture, which should alter glutamate-neurons' activation. And I do not...

22 February 2023 6,016 2 View

The reason for the low activity of Cas proteins in eukaryotes?

20 February 2023 5,194 1 View

Merging two subunits in one functional enzyme in Chimera?

Hello! I would very much appreciate some help. I have the 3d structure prediction of subunits alpha and beta of a dioxygenase from Phyre2 and I would like to merge these components in a final...

20 February 2023 145 3 View

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