We are exploring possibilities to adapt our stereo microscope due to the high cost of fluorescence stereomicroscopes.
I recently came across this product (https://nightsea.com/products/stereomicroscope-fluorescence-adapter/). However, I've been unable to find any independent reviews or images taken with this particular adaptation that are not provided by the company itself.
In our research, we inject mutations fused to fluorescence proteins such as RFP and GFP in zebrafish embryo. It would greatly assist us in our work to be able to sort embryos based on their expression levels and capture full-body images of larvae under fluorescence. Has anyone attempted to use this adapter and could provide insight on its effectiveness?
Anyone can tell me if it's really worth it?
I have used it to sort transgenic fish and find it quite useful for that. The only issue is you do have to fiddle with it some to make sure the light source is properly positioned, but once you have it in place you can see things quite well. I'm not sure how well it work for images as that's not something I use it for.
Hello!
I’m using it for my research frequently. I’m studying fluorescence in coral-dwelling gall crabs that are below 1 cm in size. I think it is a great system that gives you the freedom to try different excitation and filter settings. I had two custom made little metal stands built to adjust light positioning but you can do this in every little workshop dyi. I was able to capture fluorescent images of my specimen that were then used to determine the fluorescent area on my crabs in ImageJ (as one possible application). Let me know if you have further questions about the system!
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