Fluorescence

The property of emitting radiation while being irradiated. The radiation emitted is usually of longer wavelength than that incident or absorbed,... | Contact experts in Fluorescence to get answers

2,028 views 2,836 posts

Questions related to Fluorescence

How to calculate activation energy of OER using Tafel plot at different temperature?

How to calculate the activation energy of OER using Tafel plot at different temperatures? I have been constructing the Tafel plots at different temperatures for OER reaction. I wonder how to...

30 November 2022 7,668 3 View

Can anyone provide UV-vis spectra of phenol in water and ethanol ?

I have searched for a long time and have not found the UV-Vis spectrum of ethanol in the formally published paper, can someone help me please.

29 November 2022 9,066 7 View

Hi. How do I calculate animal (non-clinical) sample size?

Is there a similar calculator to epitools?

23 November 2022 1,490 3 View

Which brand and REF number of Collagenase do you recommend for skin biopsy digestion?

Dear colleagues, I am currently trying to set up a protocol for skin biopsy processing with the aim of obtaining fibroblasts for SCNT research in animals. Does anyone have a recommendation...

22 November 2022 9,138 0 View

Photokilling experiments: why is it called "time-dose reciprocity law" ?

Hello, in photokilling experiments (e. g. UV + bacteria models), we often refer to the so called "time-dose reciprocity law", which applies with some limitations (which I wouln't like to consider...

21 November 2022 8,765 0 View

How to solve high RMSD problem for calculation of Protein-Ligand RMSD?

I ran a MD simulation for 30 ns using Amber99ff ILDN for protein forcefield and GAFF for ligand forcefield for GKRP and F1P (PDB: 4BB9) consecutively. However, the result shows that the RMSD...

18 November 2022 6,244 3 View

Is there any collaborative relationship between plant tissu culture and viruses?

I want to understand if there is collaborative relationship between Plant tissue culture and viruses if there is what are them

16 November 2022 2,335 2 View

What could be the probable reason for dual emission as discussed below?

I have an oxindole derived compound. To study its photophysics the compd was dissolved in DCM with varying concentrations but when emission was recorded it was observed that at low concentration...

16 November 2022 4,269 2 View

How to avoid agglomeration of carbon dots?

Hello, I have been working on the synthesis of carbon dots (CDs) by electrochemical method, and I have been using monoethylene glycol as a surfactant agent, to avoid agglomeration of the CDs,...

14 November 2022 9,446 7 View

Could someone tell me what software is used here?

Hello! As you could see, I am very interested in this file from DOI: 10.1021/acscatal.5b02751: alignment of protein sequences and corresponding aa dominance were carried out in the attached plot....

14 November 2022 4,306 1 View

Can any one give reason why I am getting gram positive bacteria in MRS media ?

I am examining the presence of lactobacillus in a commercially available kombucha sample. Following serial dilution upto 10^3 because above that dilution no growth was observed. I had tried the...

12 November 2022 390 1 View

Disruption the expression of a protein which belongs to a complex must affect other proteins expression of complex?

For example, would disruption the CUL4A or DDB1 or CRBN expression of the E3 ubiquitin ligase CUL4-RBX1-DDB1-CRBN (known as CRL4(CRBN)) affect other proteins expression of this complex?

10 November 2022 713 0 View

How to perform Western blot on low cell count?

Hello I am working on a protocol to perform Western Blot on sorted endothelial cells from the liver. So far, I tried to do protein isolation in NP-40 buffer with 120.000 cells but I do not get...

08 November 2022 8,521 4 View

RDF Calculation of water ?

Hi, I have a water simulation box with 116 water molecules. I used TIP4P water model. Now I need to calculate RDF of water-water, RDF of Oxygen-Oxygen and RDF of Hydrogen-Oxygen. I tried to...

06 November 2022 6,361 1 View

How to elute enzyme protein and maintain the enzyme activity in the elution process by GFP-trap agarose kit (chromotek)?

Dear all, I have some questions about the elution process of GFP-trap agarose. What's the best way to elute the protein and keep the enzyme activity at the same time since the eluted protein need...

01 November 2022 9,729 5 View

How to calculate At%, Wt% and mol% of Dopants in Nanoparticle Synthesis in any methods?

For Example, 1 At% / 1 Wt% / 1 mol% of Zn doped in TiO2.

31 October 2022 6,789 4 View

Why does the 30min dark phase (adsorption) create asharp curve and 1hr light phase show a close to linear shape? is the photocatalyst less efficient?

0.1mg/l is used to degrade 10mg/l solution(RhB) under white light irradiation at room temperature, ph 3 and contineous magnetic stirring during irradiation in doubled layered glass reactor with...

31 October 2022 3,898 2 View

How long to keep frozen PBMC (-80°C) without change of activity ?

Hello to everyone, I work with PBMC from healthy donors extracted by ficol. I do experiments where I measure the activity of PBMC - adcc activity - capacity of spheroid infiltration I have...

27 October 2022 9,519 0 View

Problem with Rietveld refinement for .irf in Fullprof?

Dear Reader, I am trying to perform Rietveld refinement with irf function for crystalline nanoparticles. During the refinemnt, I did not get results for the volume and compression shown in the...

25 October 2022 6,518 1 View

How to make 2% of nitric acid solution in 100ml of water?

Hello please assist, How can I make a 2% of nitric acid solution in 100ml of water? if you don't mind please show with calculation so I to understand better. Thanks.

20 October 2022 7,265 4 View

Alamar blue proliferation assay at 570 nm gives same reading for different cell number. Why this is happening?

I have added different number of cells (raji cell line) to wells of adjacent columns of 96 well plate starting from 3000 to 90000 cells. But the reading of all wells at 570 is same. For reference...

19 October 2022 3,201 3 View

Pipetting affects T cell activation?

Hi, I'm activating CD8 T cells by adding MHC-I restricted epitopes to whole PBMCs in vitro according to previous published studies. I noticed that cell feeding occurs 24 hrs after adding peptides...

19 October 2022 3,411 0 View

How to innoculate ectomycorrhizal spore suspention on test Seedlings to observe growth variation?

I have collected several host-specific ecto-mycorrhizal samples from the field and I am willing to inoculate them (Spore suspension) into the same host seedlings to observe growth variation...

17 October 2022 6,311 4 View

Dextramer vs tetramer?

Hi, I'm trying to stain tumor specific TCR on T cells with either tetramer or dextramer. From what I know about these two techniques, dextramer helps to detect more tumor specific T cells due to...

12 October 2022 6,715 0 View

Badges
Science topic