I have isolated temperate phages who are forming turbid plaques. I am facing difficulty while concentrating them
Do you have any information about what family of phage they are from? Some temperate phages can be chemically induced to enter the lytic part of their lifecycle. Have you tried different culture times/temperatures?
Most temperate phages will still give reasonable lysates and titers. The fact that you can see a plaque tells you that some fraction of the host cells are lysing and producing phage. But it will certainly be somewhat lower than for a lytic phage. As Robert Adolf Brinzer mentions you should try different conditions, especially MOI's and length of time of growth of the culture. Adding an inducing agent (generally mitomycin C for many but not all phages) is another way that you might increase the titer.
Robert Adolf Brinzer Thank you for your response. Its a mycobacteriophage. I can see plaque but while concentrating them they are not that much concentrated.
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