Dear Itay

So what did you get after performing all of that? I mean, you performed correct steps, so you should already have a method for citrate detection. Can you please specify your question?

Albert Batushansky

For some reason I can't detect the molecule, I thought maybe my method worng.

Maybe it is better to run it with column, what column will be best?

Dear Itay

There are several methods for citric acid. Probably, the simplest one will be RP chromatography with HSS T3 or similar column. The retention in not the best, but acceptable. For the optimized method, I would use HILIC based methods, but again - it can take some time and efforts to develop it.

I am, however, more concerned that you do not see it with direct injection... do you work with a pure standard? What kind of instrument do you use?

Albert Batushansky

I work with standard, the instrument is xevo g2-xs QTof.

I will ask my technician.

Thanks again.