Fluorescence

The property of emitting radiation while being irradiated. The radiation emitted is usually of longer wavelength than that incident or absorbed,... | Contact experts in Fluorescence to get answers

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Questions related to Fluorescence

How to propagate temperate phages? Are they supposed to stay with the host itself during purification?

I have isolated temperate phages who are forming turbid plaques. I am facing difficulty while concentrating them

09 May 2023 8,367 3 View

In the dual luciferase reporter assay the fluorescence value of firefly is around 300 to 800. Is it lower than the detection limit of Promega Glomax?

I'm doing a stable expression of dual luciferase assay system with one vector. But I'm getting low firefly fluorescence value, around 300 to 800, and my renilla fluorescence value is around 10000...

09 May 2023 7,371 1 View

How can I plot ring current density maps using a software in windows machine?

How can I plot ring current density maps using a software in windows machine? It will be of great help if someone can let me know about the steps to this effect, for example in AIMALL software.

08 May 2023 5,745 2 View

How can I detach my mesenchymal stem cells from flask?

Hello everyone. About three months ago, I could detach my mesenchymal stem cells obtained from 8-week-old mice with trypsin %0.25 on the 10th day in 3-4 minutes. However, now I can not detach the...

04 May 2023 6,131 5 View

How do you make the AKTA Pure connect to the F9-C fraction collector?

I recently reactivated our AKTA Pure (after we moved it to a new bench), but the AKTA is suddenly not recognizing the F9-C fraction collector. (It was working well before.) I can hear the fraction...

02 May 2023 8,470 2 View

Live cell imaging with both BODIPY493/503 and LysotrackerRed?

I am wondering lipolysis on time lapse imaging in vitro. as i think if bodipy493/503 which stains Lipid droplet or neutral lipid may colocalize with lysotracker red signal i would be able to think...

01 May 2023 9,946 0 View

Which method to use for dealing with missing data (MCAR)?

My study has the following details: N=130 3 Conditions 5 Timepoints 6 Variables (measured at every time point) Analysing using Little's MCAR test revealed that my data is missing completely at...

30 April 2023 3,825 0 View

What could be the reasons if a fluorophore is giving fluorescence on another wavelength instead of its own w avelength??

In the fluorescence imaging, I am getting fluorescence at 800 nm instead of 690 nm. What could be the reasons behind this??

29 April 2023 9,064 3 View

Not getting positive colonies?

I have been trying to clone a coding region of my gene in pdonr using gateway method. I successfully cloned CDS but when I sequenced the colony the region from the middle of about 100 BP was...

24 April 2023 5,499 2 View

How to surface immobilize gold nanotriangle on glass substrate??

I tried to immobilize CTAC capped gold nanotriangle which is synthesized by seed mediated growth method by various method. (1) Glass coverslip was cleaned by: 1N NaOH, acetone, methanol, milli-q...

24 April 2023 7,005 0 View

Is it possible to reuse used cellulose membranes? Is there a proper procedure?

I want to reuse the cellulose membranes I used in the experiments. Can you help me find a procedure on this?

24 April 2023 9,919 0 View

Graphite is an conductor of electric current or a semiconductor ?

Graphite is an allotropic modification of carbon, therefore it is a non-metal. In general, non-metals do not conduct electricity. Carbon is from the same group as silicon and germanium. It can be...

19 April 2023 4,746 3 View

How to experimentally quantify the binding affinity between molecules and metal surface?

I have two metal complexes which have different affinity to Cu nanoparticles. Now I can only check this difference by naked eye, since one of them will induce much more aggregations of Cu...

13 April 2023 7,247 1 View

What are the most prominent problems that chemistry laboratories in secondary schools suffer from?

give me 3 problems

12 April 2023 6,160 5 View

Failed In-situ hybridization due to Formamide degradation?

Hi Everyone, In the past couple of months, I have been trying to do in-situ hybridisation of some mounted fish brain slides, and I have had huge problems on day two after the 50% formamide:2xSSC...

11 April 2023 8,713 2 View

What are the IC50 ranges of the following chemo drugs Cytarabine , methotrexate, Fluorouracil , cyclophosphamide, Pacitaxel, cisplatin on MCF7 ?

Does it change based on the incubation period of the drug with the cells 48hrs versus 72 hrs ?

11 April 2023 3,547 0 View

How to measure the surface hydrophobicity of protein sample with fluorescence spectrometer after i get the data of wavelength scan?

why do we use multiple concentrations of 0.05, 0.10, 0.15 and 0.20 mg/ml? after i get the overall data of scanning, what is the next step to get the final results for hydrophobicity? I am using...

10 April 2023 8,662 2 View

Cell that were resistant to virus infection but a lot of cells died after incubated with virus?

HeLa cells were resistant to TGEV infection. However, when I incubate the Hela cells with about 1 MOI of TGEV, a lot of cells died two days after incubation. Why does this happen? is it due to the...

10 April 2023 5,231 4 View

What is the absorbance and refractive index value of strontium hexaferrite?

Hello everyone, can anyone help me with the following What is the absorbance and refractive index value of strontium hexaferrite? If someone has calculated them I would appreciate it if you...

07 April 2023 362 1 View

TIM1+G sustitution model in MEGA?

How could I run a Phylogeny Reconstruction analises using Maximum Likelihood method in MEGA 7 with a TIM1+G sustitution model? Is it possible?

05 April 2023 9,365 0 View

What tools can i use to analyze metabolic pathway of a bacteria to check if it can be used to express a foreign metabolite of interest?

I am trying to metabolic engineer Salmonella typhimurium to synthesize a molecule by interest, How can i analyse the pathways of Salmonella typhimurium for the efficient production of this...

03 April 2023 5,794 1 View

Can we use CD spectroscopy for the study of Interaction of single stranded DNA with metal complexes??

Can we use CD spectra for Interaction of G6 with NiCl2??

03 April 2023 225 1 View

How to get optical constants of Tantalum for using as plasmonic material in PCF Sensor?

I have been working on PCF & Plasmonic Materials and for simulation I am using COMSOL Multiphysics. In my PCF design I am using Tantalum as plasmonic material. But the problem is how do I use...

01 April 2023 8,081 2 View

Geneious - Mauve - How to display sequence name and position?

In the original version of Mauve, when you scroll through the alignment it tells you the contig/chromosome your mouse is over and the exact position at the bottom of the window. The Mauve aligner...

31 March 2023 4,549 0 View

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