I have carried out an 18s rna V7 eDNA analysis on marine sediments to look for Macroalgal contribution. The data has provided data but the sequnce searches on NCBI blast are not very conclusive other than identifying phylum to family level and I have been trying to look in the Silva 18Srna database to try to see if there is anything more divined to Genus or even hopefully species. I know at 100-110bp in V7 out of the whole 18S there isnt much scope to sharpen this up but I need to do this.
Problem is I dont really know how to use Silva Ive run a few sequences but I dont know if what I have done is correct and the results are valid info for what I want.
unfortunately the tutorial within silva is slab text and as I have ADHD I am a more visual learner so its not particularly user friendly for me. Does anyone know/have links to youtube video that shows a noob user the basics of running Silva searches?