Fluorescence

The property of emitting radiation while being irradiated. The radiation emitted is usually of longer wavelength than that incident or absorbed,... | Contact experts in Fluorescence to get answers

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Questions related to Fluorescence

How does population density affect population growth and how population distribution and density can impact the environment?

24 October 2023 2,317 5 View

Any vacant position for PhD in Plant Ecology?

Hi, Worldwide Researchers, My name is Arshad Rasool. Currently, working as a botany lecturer at university of central punjab, Lahore.

18 October 2023 3,775 5 View

Correction of ELISA sputum cytokines/chemokine supernatant results?

Dear all, I am looking at analytes in sputum supernatants via ELISA (MSD). When sputum is processed in our lab, 8x the weight of the plug in PBS is added to the plug before dispersion and then...

18 October 2023 7,909 0 View

What are the effects of pH on Fluorescence and Absorption of Flavins (Riboflavin, FMN, FAD) and NADH/NADPH in a Simple Aqueous Solution?

Dear all, I am interested in understanding the effects of pH on the spectrophotometric behavior of flavins (riboflavin, FMN, and FAD) and NADH/NADPH (including the oxidized form). Specifically, I...

15 October 2023 4,917 2 View

How to measure the photodetection property of a bulk powder material? what are the instruments and techniques required for this?

I want to perform Photodetection studies of my bulk powder sample, but don't have any idea where to start. so it will be very helpful for me if someone who knows about this topic can give some...

09 October 2023 3,112 1 View

Why do the colonies of the fungus turn yellow when Trichoderma strains are grown on a solid agar (wort agar) medium?

does it depend on the components of the environment or the influence of physical factors (light)?

05 October 2023 9,120 0 View

What are the factors on which we can decide where to put miniscrews for better result of opening the palatine suture in adults?

what are the factors on which we can decide where to put miniscrews for better result of opening the palatine suture in adults

30 September 2023 1,824 0 View

Lymphedema post breast cancer?

What are appropriate scale that used in measuring lymphedema progress during and post chemotherapy management specially in Theraputic exercise

28 September 2023 3,541 0 View

What can be the other possibilities of not getting any colonies after transformation if the efficiency of competent cells is low?

I am doing Pcr cloning trying to clone a 3000 bp pcr product into a 9000 bp pcdh_bec1 plasmid .digestion was done using Xba1 and Not1 ,after gel extraction loaded 2ul on the gel and observed faint...

28 September 2023 3,092 2 View

Is it possible to do an statistical test to compare single integration versus multiple integrations of related endogenous retroviruses?

Hello, I am working with closely related endogenous retrovirus (ERV) sequences. I suspect these might have been the result of several integration events from different but related exogenous...

26 September 2023 7,008 3 View

Production of greenhouse glass and defuse glass ??

Hi If you have any information or experience regarding the production of greenhouse glass and what standards the produced glass should have, please let me know. Thank you in advance for your time.

25 September 2023 3,655 2 View

Help with GFP and ChAT co-staining?

I am trying to co-stain for GFP and a ChAT marker in the brain of a mouse model that has GFP-expressing neurons. Typically I get a strong fluorescent signal when I stain only for GFP (photo 1),...

19 September 2023 5,839 2 View

I am unable to amplify my 3.8kb gene from cDNA of Drosophila?

The Tm of primers is 65 degrees and I am getting only non specific bands around 1kb. I amplified using Q5-HF Polymerase. How can I improve the specificity to get my desired band?

18 September 2023 4,927 5 View

How to prepare cell lysis for Nf1 CRISPR/cas9 knockout HeLa cells?

I have Neurofibromin knockout HeLa cells. The cells won't lyse properly and the western blot always comes out with smears or uneven bands. I have tried 1x RIPA buffer for lysis and shearing and...

18 September 2023 3,534 3 View

The reson for the absence of band in gel?

Hellow fellow academics I am currently in a dilemma and I would really appreciate some suggestions/guidance on the matter. Situation: I have overexpressed my gene of interest (GOI) from wheat in...

17 September 2023 5,186 6 View

How to insert statictical lettering A,BC... both capital and small supercripts... showing significance in multiple factors?

The picture i uploaded has supercripts with capital and lowercase letters, both indicating diferent significance level. Can someone explain this?

16 September 2023 3,103 0 View

Dose anyone know what is the optimum N2a cell # for LDH assay?

This is Graph of N2a cell of LDH activity vs. N2a cell number. What is the optimum cell number for LDH assay? 1. Absorbance number is not higher than 2? In this case is 5000 or 2. Before reach...

15 September 2023 2,728 2 View

Retinitis pigmentosa study proposal?

Would anyone like to do a before and after visual field study on me and a few others with RP? The Wellspring Clinic in Vancouver Canada is helping a lot of people that I know via accupuncture i...

12 September 2023 3,989 0 View

Why my Ni structure is not converging after applying a strain in X-direction?

Please find the attached Ni Quantum Espresso Input file. &CONTROL calculation = 'scf' verbosity = 'high' restart_mode = 'from_scratch' tstress = .true....

12 September 2023 9,901 0 View

Does the x axis of the biphasic dose response function has to be logarithmic?

I performed the ITC (isothermal titration calorimetry) experiments by titrating the LUVs made of POPC and POPG into the daptomycin solution. After exporting the data in the Excel, I used the...

08 September 2023 7,566 2 View

Can alkyl diazirine fluorescent probes label BSA without light activation?

I am performing an in vitro in-gel labeling experiment by photo-activating a fluorescent alkyl diazirine probe with UV light in the presence of BSA to determine the required activation parameters...

08 September 2023 5,110 0 View

Sensors in Formula student racing?

Hello, as part of formula student car racing I need a SLAM algorithm that will recognize cones and will build a map (there will be a few laps therefore map is needed for optimal path). The cones...

04 September 2023 1,703 1 View

How to interpret the following RMSDS?

Hi everyone. So I have performed MD simulations on desmond of my protein bound to a mutant peptide? My objective is to elaborate how the mutant peptide is inducing conformational changes in the...

22 August 2023 7,088 1 View

Can anyone help me with phonon calculation in quantum espresso?

I am facing problems in doing phonon calculations in quantum espresso. If I could have a sample code and information about the Pseudopotential file, it could be a great help for me.

19 August 2023 2,391 0 View

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