Fluorescence

The property of emitting radiation while being irradiated. The radiation emitted is usually of longer wavelength than that incident or absorbed,... | Contact experts in Fluorescence to get answers

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Questions related to Fluorescence

What is the protocol and phyto-sanitary certification for seaweed export/import?

I am looking for protocol and phyto-sanitary certification being issued for seaweed export/import by different countries. If there is any specific information, please share. I am

16 August 2024 7,853 0 View

What causes unspecific binding of isotype ctrl Ab to my organoid cells and how to counteract it?

I have attempted to stain patient-derived cancer organoid cells that have been brought into suspension by accutase and trypsin treatment (for a normal split) for surface markers. I wanted to...

07 August 2024 3,628 0 View

How to differentiate electron transfer control step or chemical reaction control step using experiments?

For an electrode electron transfer reaction with a subsequent chemical reaction, there are the some method to differentiate electron transfer control step or chemical reaction control step using...

01 July 2024 6,953 0 View

Laser Capture Microdissection using Zeiss Palm microbeam instrument ..?

Dear Researchgate community. Is anyone doing laser capture microdissection using the Zeiss Palm microbeam instrument? If so, do you have an alternate (non-Zeiss) source for 500uL adhesive...

07 June 2024 5,210 2 View

Best hydroxyl vs acetate vs chloride salt based peptide pool for T cell stimulation?

I would like to use a peptide pool to stimulate lymphocyte/T cell to check recall responses. Most of the peptide pool come as TFA salt but there are reports that suggest TFA salt based peptide...

24 May 2024 5,251 0 View

How should dexmedetomidine be monitored in clinical practice, and what vital signs are particularly important to observe?

How should dexmedetomidine be monitored in clinical practice, and what vital signs are particularly important to observe?Dexmedetomidine: A Comprehensive Review of Pharmacology, Cli...

09 May 2024 1,020 2 View

Which long preservation method(s) can I use for Stenocarpella maydis also Diplodia maydis? So that I remains intact and does not loose traits?

I am Senior Research Technician and I have some isolates of Stenocarpella maydis also Diplodia maydis. I am currently try to develop SOP for long term preservation, so that it does not loose its...

07 May 2024 5,865 4 View

D-dimer sensitivity as a biomarker for various cancerous tumors?

Is D-dimer elevated with tumor cells?

01 May 2024 8,385 1 View

Warning and error DMol3 message?

hi people, I am doing a geo optimization in DMol3, the calculation seems to converged, but I got a warning and error message like the following, can someone explain me what that means? and what...

30 April 2024 6,782 4 View

How can the slicing parameters be transpolated from Leica VT1200 to VT1000??

I´m planning to make some experiment using rat liver slice cultures, but I´m facing some troubles. Where I learned the protocol, I used a Leica VT1200 and the parameter for cutting were...

18 April 2024 3,900 0 View

What products are produced from the decomposition of methyldiethanolamine (MDEA) in water???

08 April 2024 4,892 0 View

Is it possible to produce a very small amount of volatile oil along with condensed gas? If so, could you please provide a real example?

Is it possible to produce a very small amount of volatile oil along with condensed gas? If so, could you please provide a real example? Thank you.

05 April 2024 1,451 2 View

My plasmid DNA is 96ng. How do i calculate the volume of bsa I (20000 U) to use to digest it?

I am trying to do a golden gate cloning but the ligation-digestion seems to be failing

02 April 2024 6,463 2 View

Which statistical test should I use?

Hi, I need some information. After collecting data from various experiments, I am proceeding with a statistical analysis. Since I work with frequencies, I was told to use the chi-square test....

21 March 2024 5,525 4 View

What's best conductor of electricity &increasing temperature increasespecific resistivity onconductors as decreasespecific resistanceof semiconductor?

What is the best conductor of electricity and why does increasing temperature increase the specific resistivity on conductors whereas decrease the specific resistance of semiconductors?

19 February 2024 9,586 7 View

How can I prepare 3 amino benzoyl chloride from 3 amino benzoic acid and thoinyl chloride and which method and what solvent I have to use ?

How can I prepare 3 amino benzoyl chloride from 3 amino benzoic acid and thoinyl chloride and by which method and what solvent I should use ?

16 February 2024 4,896 0 View

How can I prepare 3 amino benzoyl chloride from 3 amino benzoic acid and thoinyl chloride and which method and what solvent I have to use ?

How can I prepare 3 amino benzoyl chloride from 3 amino benzoic acid and thoinyl chloride by which method and what solvent should I use ?

16 February 2024 5,366 0 View

What are the non financial investment decisions indicators?

There are different types of investments; Equity, fixed income such as bonds, alternative investments such as real estate, mutual funds, and unit trusts among others. A good investment decision is...

06 February 2024 1,914 4 View

Insuline resistance in HepG2 cells?

Can you please tell me how you induce insuline resistance. My protocol does not work. Seems like i'm missing something. i seed my cells at 1 million in petri culture and starved them for 24h. I...

17 January 2024 5,805 1 View

How can I find the crystallographic information file (CIF) for Nylon 6 or 6/6 (Polyamide)?

polyamide 6

13 January 2024 7,641 0 View

What electroporation parameters work best to viably transfect a 10kb plasmid into CaSki cells with high efficiency?

Dear colleagues, I am planning to transfect a 10 kb plasmid into Caski cells using electroporation. I would greatly appreciate if someone could share a detailed protocol for electroporating...

12 January 2024 2,760 0 View

How do forests help to maintain the balance of gases in the air and plants maintain the balance of oxygen and carbon dioxide in the atmosphere?

09 January 2024 7,477 2 View

Is a 5` phosphate needed for homologous recombination?

I am a bit confused whether phosphorylated DNA is needed to achieve efficient homologous recombination in e coli. I believe there are methods that use ssDNA oligos or PCR products as inserts for...

05 January 2024 2,216 1 View

I need help interpreting my BCA results. I don't understand what these values mean?

Good morning. I recently performed a BCA, and I obtained the following values for my protein concentration. I formula I used in Excel was: =(E39-0.0798)/0.0377 I then multiplied the value by 10 or...

02 January 2024 3,224 3 View

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