Fluorescence

The property of emitting radiation while being irradiated. The radiation emitted is usually of longer wavelength than that incident or absorbed,... | Contact experts in Fluorescence to get answers

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Questions related to Fluorescence

Laser Capture Microdissection using Zeiss Palm microbeam instrument ..?

Dear Researchgate community. Is anyone doing laser capture microdissection using the Zeiss Palm microbeam instrument? If so, do you have an alternate (non-Zeiss) source for 500uL adhesive...

07 June 2024 5,150 2 View

Best hydroxyl vs acetate vs chloride salt based peptide pool for T cell stimulation?

I would like to use a peptide pool to stimulate lymphocyte/T cell to check recall responses. Most of the peptide pool come as TFA salt but there are reports that suggest TFA salt based peptide...

24 May 2024 5,243 0 View

How should dexmedetomidine be monitored in clinical practice, and what vital signs are particularly important to observe?

How should dexmedetomidine be monitored in clinical practice, and what vital signs are particularly important to observe?Dexmedetomidine: A Comprehensive Review of Pharmacology, Cli...

09 May 2024 1,013 2 View

Which long preservation method(s) can I use for Stenocarpella maydis also Diplodia maydis? So that I remains intact and does not loose traits?

I am Senior Research Technician and I have some isolates of Stenocarpella maydis also Diplodia maydis. I am currently try to develop SOP for long term preservation, so that it does not loose its...

07 May 2024 5,832 4 View

D-dimer sensitivity as a biomarker for various cancerous tumors?

Is D-dimer elevated with tumor cells?

01 May 2024 8,377 1 View

Warning and error DMol3 message?

hi people, I am doing a geo optimization in DMol3, the calculation seems to converged, but I got a warning and error message like the following, can someone explain me what that means? and what...

30 April 2024 6,760 4 View

Is it possible to produce a very small amount of volatile oil along with condensed gas? If so, could you please provide a real example?

Is it possible to produce a very small amount of volatile oil along with condensed gas? If so, could you please provide a real example? Thank you.

05 April 2024 1,434 2 View

My plasmid DNA is 96ng. How do i calculate the volume of bsa I (20000 U) to use to digest it?

I am trying to do a golden gate cloning but the ligation-digestion seems to be failing

02 April 2024 6,441 2 View

Which statistical test should I use?

Hi, I need some information. After collecting data from various experiments, I am proceeding with a statistical analysis. Since I work with frequencies, I was told to use the chi-square test....

21 March 2024 5,521 4 View

What's best conductor of electricity &increasing temperature increasespecific resistivity onconductors as decreasespecific resistanceof semiconductor?

What is the best conductor of electricity and why does increasing temperature increase the specific resistivity on conductors whereas decrease the specific resistance of semiconductors?

19 February 2024 9,577 7 View

How can I prepare 3 amino benzoyl chloride from 3 amino benzoic acid and thoinyl chloride and which method and what solvent I have to use ?

How can I prepare 3 amino benzoyl chloride from 3 amino benzoic acid and thoinyl chloride and by which method and what solvent I should use ?

16 February 2024 4,867 0 View

How can I prepare 3 amino benzoyl chloride from 3 amino benzoic acid and thoinyl chloride and which method and what solvent I have to use ?

How can I prepare 3 amino benzoyl chloride from 3 amino benzoic acid and thoinyl chloride by which method and what solvent should I use ?

16 February 2024 5,337 0 View

What are the non financial investment decisions indicators?

There are different types of investments; Equity, fixed income such as bonds, alternative investments such as real estate, mutual funds, and unit trusts among others. A good investment decision is...

06 February 2024 1,906 4 View

Insuline resistance in HepG2 cells?

Can you please tell me how you induce insuline resistance. My protocol does not work. Seems like i'm missing something. i seed my cells at 1 million in petri culture and starved them for 24h. I...

17 January 2024 5,797 1 View

How can I find the crystallographic information file (CIF) for Nylon 6 or 6/6 (Polyamide)?

polyamide 6

13 January 2024 7,636 0 View

What electroporation parameters work best to viably transfect a 10kb plasmid into CaSki cells with high efficiency?

Dear colleagues, I am planning to transfect a 10 kb plasmid into Caski cells using electroporation. I would greatly appreciate if someone could share a detailed protocol for electroporating...

12 January 2024 2,754 0 View

How do forests help to maintain the balance of gases in the air and plants maintain the balance of oxygen and carbon dioxide in the atmosphere?

09 January 2024 7,466 2 View

Is a 5` phosphate needed for homologous recombination?

I am a bit confused whether phosphorylated DNA is needed to achieve efficient homologous recombination in e coli. I believe there are methods that use ssDNA oligos or PCR products as inserts for...

05 January 2024 2,209 1 View

I need help interpreting my BCA results. I don't understand what these values mean?

Good morning. I recently performed a BCA, and I obtained the following values for my protein concentration. I formula I used in Excel was: =(E39-0.0798)/0.0377 I then multiplied the value by 10 or...

02 January 2024 3,219 3 View

What organisms are involved in soil structure fertility and role of microorganisms in decomposition and soil fertility?

28 December 2023 8,274 0 View

Can I determine the Optical Limiting Threshold from Z-scan experimental data?

...

22 December 2023 1,256 3 View

What are some well studied membrane-localized receptors, or constructs, with different or tunable dimerization affinities?

I would like to use them as controls. I am studying the dynamics of receptors on the cell surface using SMI TIRF microscopy. I am currently using CHO cells and I would like to use constructs as...

20 December 2023 3,272 0 View

How to change node-edge binding position in Cytoscape Desktop?

Dear colleagues, I am trying to plot a protein-protein interaction network in Cytoscape. My idea is to place the node representing the bait protein on one side of the figure, and on the other...

19 December 2023 9,204 2 View

Can we culture the human PBMC with nanoparticle and do the metabalomics studies?

I want to know, can we culture the human PBMC with nanoparticle and do the metabalomics studies? Please let me know

16 December 2023 571 2 View

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