Fluorescence

The property of emitting radiation while being irradiated. The radiation emitted is usually of longer wavelength than that incident or absorbed,... | Contact experts in Fluorescence to get answers

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Questions related to Fluorescence

What is the best order for CV, GCD, and EIS testing in electrochemical tests of supercapacitors?

I am not sure about the order of CV, GCD, and EIS in electrochemical testing of supercapacitors.

3 weeks ago 1,623 0 View

How carbon quantum dots be used for cancer therapy?

CQDs are quasispherical carbon structures with sizes less than 10 nm, in which core carbon atoms are sp2-hybridized. As quantum dots, their excellent photoluminescence, good water solubility,...

4 weeks ago 727 2 View

Hello colleagues! I need some help/assistance. Can anybody tell which Caudovirales family does this phage belong?

Your ideas and explanation would be highly appreciated at this point. The phages in the TEM image are from the same sample and processed using JEM 2100 transmission electron microscope.

last month 3,941 1 View

Looking to get chapters from an experienced seaweed specialist(s) for the edited book from CABI ?

Dear Researchers, Good day and Warm Greetings from Central Salt and Marine Chemicals Research Institute, India! I am pleased to inform you that, CABI, UK based inter-governmental organisation...

last month 1,930 1 View

Why reorientation/dislocation of atom after optimization occurs ? How to fix this problem?

I am doing geometry optimization of a large molecule containing an alkyl group and a anionic part contain SO4Na. But after geometry optimization I received a structure where oxygen atom got...

last month 9,594 11 View

During cruroraphy for hiatal hernia, how do you decide where to place the sutures - above or below the OG junction?

Traditionally, the crura are sutured together below the OG junction. However, the location of the hiatal hernia is always above the OG junction. So should we not perform the cruroraphy above the...

06 August 2025 2,501 5 View

In synthesizing Graphene quantum dots from rich straw i am getting instead of solution with GQDs i got dried black powder. How to get GQDs from it?

Procdure: Initially, 2.0 g of paddy straw was taken and fine powder was obtained by mechanically grinding. The powder was then washed with 0.15 M HCl and heated at 50 ◦C for 3 h. To synthesize the...

03 June 2025 4,106 2 View

Seeking advice on single-cell isolation from RNAlater-fixed tissues for scRNAseq?

Hi everyone, I am currently working with mouse skin and lung tissues preserved in RNAlater (Invitrogen). However, I have limited experience with single-cell RNA sequencing (scRNAseq). I would...

24 May 2025 1,945 1 View

How far this synthesis method is valid , reduced graphene oxide is obatined from dead neem leaves , without any addition of chemicals or processing?

How far this synthesis method is valid , reduced graphene oxide is obatined from dead neem leaves , without any addition of chemicals or processing

30 April 2025 694 0 View

Need detailed protocol for using ab112118 cytotoxicity assay kit by colorimetry?

I want to perform a cytotoxicity assay using MCF-7 cells. The kit that I use to determine cell death is colorimetric kit by abcam Cat# ab112118. The protocol says that we need to thaw the kit...

28 March 2025 8,826 1 View

Has anybody experience with E. coli SoluBL21 ?

Hi, I would like to ask if E. coli SoluBL21 cells are really so good to produce soluble proteins? I have read that they should contain extra chaperons. Will there be no problem with their removal?...

22 March 2025 7,337 2 View

Which brand brackets are you using?

Conventional twin MBT brackets. Which one do you recommend?

12 March 2025 6,753 1 View

TNA band not visible on gel ?

I extracted RNA from Nicithiana leaf samples using the triazole method and ran them on 1.2 percent agarose. Although I got RNA bands, the TNA bands were not visible. What may be the reason for this?

06 March 2025 8,993 1 View

What is the orthodontic treatment of impacted central incisor?

28 February 2025 4,661 2 View

Solvent in MIC and for antimicrobial activity?

I would like to seek your guidance regarding the use of Tween 80 and DMSO as solubilizing agents for plant extracts and essential oils in antibacterial activity assays. Would it be appropriate to...

13 February 2025 8,769 3 View

In a Kapitza pendulum does the unstable equilibrium point becomes a centre from saddle point? How to prove this?

I am trying to numerically obtain the solution of a Kapitza pendulum when the forcing parameters are tuned for stability. The zone of stability in the parameter plane is obtained using Floquet...

12 February 2025 8,234 1 View

How to extract I vs Q plot from Mastersizer 3000?

Does anyone know how to extract I (scattered intensity) vs Q (scattering vector) from Mastersizer 3000?

03 February 2025 6,151 4 View

Describe a spiral magnetic field (not to be confused with a solenoid field!) as a "stack" of toroidal vortices?

What mathematical apparatus is most convenient for describing a spiral magnetic field (not to be confused with a solenoid field!) as a "stack" of toroidal vortices corresponding to Faraday's lines...

03 February 2025 6,688 2 View

Orthodontic Biomechanics Using Clear Aligners ?

Orthodontic Biomechanics Using Clear Aligners

01 February 2025 1,013 1 View

Different type of Tnfa injection in mice , which one should I use for which purpose ?

I am studying a protein TnfaIP2 (Tnfa induced protein 2). It is directly regulated by Tnfa. I have a mice model where I can induce TnfaIP2 knock-down (via doxycyclin food). I would like to do...

29 January 2025 4,275 0 View

Marker set for colorectal cancer-associated cancer stem cells?

Dear ResearchGate Community, I am trying to establish a flow-cytometry based 3 color panel to identify cancer stem cells in patient-derived colorectal cancer organoids. So far I've seen a large...

29 January 2025 5,998 0 View

For protein extraction from tissue, what is causing to the formation of a really small pellet in freshly homogenized sample preparation?

I am facing some trouble while pelleting tissue debris. I am using the attached protocol. After centrifugation and removing supernatant, I store my supernatant in -80c until bca estimation which...

18 January 2025 1,095 5 View

What are the Guidelines for Import of Live Seaweeds in different countries?

This is to inform you that “MINISTRY OF FISHERIES, ANIMAL HUSBANDRY AND DAIRYING”, (Department of Fisheries), Government of India has recently, brought out the “NOTIFICATION” on “Guidelines for...

16 January 2025 2,410 0 View

Algae Culture institutes/labs and techniques?

1. Which institutes/labs in India (Gujarat and others) have facilities for culturing algae, especially freshwater algae or sending the samples for freshwater algae culture? 2. Is the requirement...

29 December 2024 4,335 1 View

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