Fluorescence

The property of emitting radiation while being irradiated. The radiation emitted is usually of longer wavelength than that incident or absorbed,... | Contact experts in Fluorescence to get answers

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Questions related to Fluorescence

How to calculate phage concentration?

Hi all. I am trying to calculate the concentration of phages purified by the PEG method. How should I proceed? I would like to express the result in nM/ml thanks

08 August 2023 9,660 4 View

How to cryopreserved human tissue samples?

Hello everyone. I would like to know if there are some contraindications in moving human tissue samples stored for more than 6 months at -80°C (with medium and DMSO), to a new temperature of...

02 August 2023 5,322 2 View

How can I calculate the sample size required for evaluating a novel immunohistochemistry (IHC) antibody?

Hi. How do I determine the appropriate sample size for a study focused on developing a new immunohistochemistry antibody to detect a breast cancer biomarker and assessing its performance...

01 August 2023 4,707 2 View

Greetings. Is there a method for estimating carbohydrates in cyanobacteria which do not uses an acid hydrolysis step or running a column?

Both phenol-sulphuric acid and anthrone method uses a acid hydrolysis step which I really want to avoid due to safety issues. Can anyone help me with this?

01 August 2023 8,486 0 View

Why am I having troubles connecting Frac-920 after tube sensor replacement?

Hi there! I just replaced tube sensor of our AKTA purifier Frac-920 and I cannot set up runs because the connection can't be made between the instrument and the fraction collector. I...

31 July 2023 448 0 View

How fluorescence quenching can happen at a lower excitation wavelength which is lower than the highest UV absorption wavelength?

In general we see that the highest absorption wavelength of a solution is the probable excitation wavelength in fluorescence. But I am seeing quenching behavior at lower excitation wavelength for...

30 July 2023 2,690 8 View

Why are my tissue samples disappearing from my coverslipped slides and how do I prevent it?

I am currently repeating the same procedures with slightly different methods, but every time, my brain tissue slices disappear from the slides within a few weeks of coverslipping them. The slides...

28 July 2023 128 3 View

How to select the pseudo potential in case multi-component alloys In Quantum Espresso?

I am going to simulate the alloy having three elements in it. I want to know how to select the Pseudo Potential for different alloy elements.

26 July 2023 7,370 1 View

Do you know any brand that sells pre labelled petri dishes?

Hello, I'm trying to find pre labelled 90mm petri dishes to be included in an automation workflow for a new biotech lab. Does anyone know a brand? I can't find one! THX Nidia

18 July 2023 7,890 4 View

is it better to use raman spectroscopy or circular dichroism in analyzing protein folding?

I am planning to do folding pattern analysis on the secondary structure of peptide.

17 July 2023 5,356 2 View

Bright/concentrated spot on the fluorescent microscopic image?

Dear RG community. I am trying to set up a fluorescent microscopic system for micro-PIV flow measurements. I am using a blue laser (473 nm) of 3mm diameter and I am expanding it using a 10X...

14 July 2023 1,926 3 View

How do Rhizobium and blue green algae increase soil fertility and which blue green algae used in rice field to increase fertility?

13 July 2023 992 6 View

What could be the reason of multiple surges (bumps) in the IRF and decay profile of the TRPL data for weak fluorescent material ?

I took an IRF using deionized water in the TCSPC system, in which the decay profile showed multiple peaks instead of smooth exponential decay.

11 July 2023 5,196 4 View

Is there ant fluorescent protein with a totally different structure than GFP?

GFP and most other FPs have 11 beta-strands, and make up the beta-barrel. Is there any FP with a totally different structure?

09 July 2023 3,190 3 View

Can there be charge transfer from solvent to solute in excited state?

I have a compound under investigation for its photophysical characteristics, and during investigation of its emission spectra with varying polarity I observed a red shift from low to high polar...

05 July 2023 8,035 3 View

What is the unit of Transition electronic dipole moment in TD-DFT using Gausian ?

The log file contains the x, y, and z components of the electric dipole moment (Au) from the ground state to the excited state. Is there a way to convert the units of this electric dipole moment...

30 June 2023 6,151 2 View

Any tips on imaging fluorescence based on pixel number rather than value w/varying intensities on image J?

Hello! 1. I am trying to compare the intensity of proteins between two groups. However, I am aware that depending on the tissue preparation including fixing, staining, munting etc & imaging...

23 June 2023 169 1 View

How can I find the txt file of JCPDS card no. 03-065-3419 and 0-065-0395?

Hi Sirs, I try to do match with some compounds but I could not find the JCPDS No. 03-065-3419 and 0-065-0395, in Xpert program can you help me plz

23 June 2023 8,681 2 View

Can we use chemically engineered biochar and chemically modified biochar as same terms?

If both are different, what would be the difference?

22 June 2023 5,619 2 View

Why operating the reaction at lower conversion than thermodynamic equilibrium ?

For Methane dry reforming reaction (DRM), why do people try to avoid operating the reaction in thermodynamic equilibrium conversion? Thank you!

19 June 2023 9,095 1 View

What should I use between primer and probe in qPCR?

I'm trying to design the qPCR method. If I already have two primers and a probe sequence, how am I going to connect the primer and probe sequence together? ( see picture) Can I use, for example,...

14 June 2023 3,832 2 View

What type of graphene or carbon nanotube is best for concrete?

I am researching several methods of adding graphene and carbon nanotubes to concrete in order to increase the strength. Many papers have said that they added graphene, but no real descriptors of...

14 June 2023 226 6 View

Western Blot trans-blot turbo transfer system of 1.5mm PAA 15%?

Hello, I have to transfer a PAA 15% of 1.5mm onto a PVDF membrane in the Biorad Trans-blot turbo transfer system. The protocol says for a mini gel to use 1.3A up to 25V for 10 min. I read about...

13 June 2023 9,638 1 View

What is the relationship between 3D printing on quality costs?

What is the relationship between 3D printing on quality costs

09 June 2023 4,762 2 View

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