Fluorescence

The property of emitting radiation while being irradiated. The radiation emitted is usually of longer wavelength than that incident or absorbed,... | Contact experts in Fluorescence to get answers

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Questions related to Fluorescence

Any tips on imaging fluorescence based on pixel number rather than value w/varying intensities on image J?

Hello! 1. I am trying to compare the intensity of proteins between two groups. However, I am aware that depending on the tissue preparation including fixing, staining, munting etc & imaging...

23 June 2023 190 1 View

How can I find the txt file of JCPDS card no. 03-065-3419 and 0-065-0395?

Hi Sirs, I try to do match with some compounds but I could not find the JCPDS No. 03-065-3419 and 0-065-0395, in Xpert program can you help me plz

23 June 2023 8,697 2 View

Can we use chemically engineered biochar and chemically modified biochar as same terms?

If both are different, what would be the difference?

22 June 2023 5,640 2 View

pergunta cancelada. Obrigada?

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21 June 2023 361 1 View

Why operating the reaction at lower conversion than thermodynamic equilibrium ?

For Methane dry reforming reaction (DRM), why do people try to avoid operating the reaction in thermodynamic equilibrium conversion? Thank you!

19 June 2023 9,112 1 View

Is there a Silva database basic user tutorial on youtube?

I have carried out an 18s rna V7 eDNA analysis on marine sediments to look for Macroalgal contribution. The data has provided data but the sequnce searches on NCBI blast are not very conclusive...

16 June 2023 3,191 1 View

What should I use between primer and probe in qPCR?

I'm trying to design the qPCR method. If I already have two primers and a probe sequence, how am I going to connect the primer and probe sequence together? ( see picture) Can I use, for example,...

14 June 2023 3,852 2 View

What type of graphene or carbon nanotube is best for concrete?

I am researching several methods of adding graphene and carbon nanotubes to concrete in order to increase the strength. Many papers have said that they added graphene, but no real descriptors of...

14 June 2023 243 6 View

Western Blot trans-blot turbo transfer system of 1.5mm PAA 15%?

Hello, I have to transfer a PAA 15% of 1.5mm onto a PVDF membrane in the Biorad Trans-blot turbo transfer system. The protocol says for a mini gel to use 1.3A up to 25V for 10 min. I read about...

13 June 2023 9,659 1 View

What is the relationship between 3D printing on quality costs?

What is the relationship between 3D printing on quality costs

09 June 2023 4,780 2 View

What is the relationship between tomoxifen treatment with chemotherapy in breast cancer?

I have a molecular biology assignment on breast cancer treatment, I hope you can help.

09 June 2023 7,279 5 View

Is there a method for analysing and normalising ELISA data by protein content from cell culture supernatants?

I am conducting a study in which I have to measure the concentration of different hormones in cell supernatant samples by means of ELISA assays. Although the seeding conditions are always...

29 May 2023 7,858 1 View

How to write a review article for the first time?

I would like to write a review article, but I have noticed that there are various types of review articles, each with its own distinct writing style. Therefore, I would like to seek the opinions...

24 May 2023 3,370 1 View

Why Magmax viral RNA isolation automation procedure is not working for my samples?

Hello all, I have been trying 5x Magmax 96 viral RNA isolation kit (AM1836-5) for extraction of total RNA and DNA samples from swab samples (assuming that the viral titer is too low). We have...

23 May 2023 3,293 1 View

What is the difference between Positive control and Housekeeping gene in copy number analysis?

I want to run copy number analysis on falciparum isolates using relative quantification. However I do not understand the function and difference between housekeeping gene and positive control in...

23 May 2023 2,479 4 View

Is there anyone experiensed with Beta-Lactamase assay activity kit?

What is the hydrolysis buffer? After running hydrolysed Nitrocefien im kinetic mode , how can I draw the standard curve ? I mean at what time set I plot the absorbances versus the Nitrocefine...

21 May 2023 4,452 10 View

Where can we find the protein sequence of a specific Human HLA allele ?

We are trying to look for specific Human HLA allelesa nd their haplotypes. But we atre having a hard time looking for the exact sequence of the haplotype that we need. Could you please let us know...

18 May 2023 1,334 3 View

How to Prove photoinduced electron transfer mechanism?

Required any process or study to prove photoinduced electron transfer mechanism other than Weller equation through electrochemical analysis and fluorescence spectroscopy.

18 May 2023 3,852 1 View

Has anyone tried Nightsea fluorescence adaptor?

We are exploring possibilities to adapt our stereo microscope due to the high cost of fluorescence stereomicroscopes. I recently came across this product...

17 May 2023 9,548 2 View

What are the best conditions to detect citrate in LC-MS?

I am trying to detect citrate in LC-MS. I ran the samples in both negative and positive modes. I performed direct injection (without a column) using an ESI ionization source. Thank you very much

17 May 2023 7,796 4 View

Collimating/focusing the output of an LED (light engine) into a 400um multimode optic fibre, what are the approaches?

Hi. I have a Lumencor Sola solid state light engine which uses a 5mm liquid light guide (LLG). I would like to use this for a spinning disk confocal setup which uses an FC port for optic fibres....

17 May 2023 2,048 2 View

What is a possible explanation to a have a color shift to yellow on a cell culture medium?

Our final project for a college course involves the expansion of PC-12 Adh cells. We have been using DMEM's culture medium supplemented with L-Glutamine, FBS and Pen/Strep antibiotics. The cells...

14 May 2023 3,104 2 View

Can anyone tell me where I can buy 4000 nm or smaller polyethylene microspheres or polyethylene nanoplastics?

4000 nm or smaller polyethylene microspheres or polyethylene nanoplastics

12 May 2023 131 2 View

What are the precautions one must take while handling CS2 (Carbon disulfide) for organic reactions?

Any handling hazards? Is it safe?

11 May 2023 8,172 3 View

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