I've been working off an RNA extraction protocol using trizol and chloroform for several months that worked beautifully until I moved on to our most important samples. After completing them, I discovered the resulting concentration was far too low to proceed. I've no idea where I went wrong but I need to do my best to salvage this somehow.
My one saving grace is that, after adding chloroform and separating out the top layer I also kept the bottom and interphase layer and stored it. My intention was to retain this for a DNA extraction later, but I'm wondering if my RNA could still in this layer and perhaps there could still be a way to extract it.
Has anybody done this before, and could they give me advice how to proceed?
Add water/buffer to what is left and complete phenol/chlorophorm extractions and precipitate with ethanol and hope that you will get something. Fingers crossed.
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- Analytical Chemistry
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