The cryopreservation protocol I am familiar with is as following:
1. PFA 4% overnight at 4C
2. WashX3 cold PBS
3. Sucrose 15% at 4C until submerged
4. Sucrose 30% at 4C overnight
5. OCT embedding
I used this protocol for cryopreservation of pancreata from mice.
Malcolm Nobre
Hello Esti Carmel
The steps are very much similar to the protocol below.
1. After removal of tumor tissue from mice, wash briefly in ice cold PBS plus Ca++ and Mg++.
2. Fix tumor tissue in fresh (
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Esti Carmel
Hello Malcolm Nobre ,
I appreciate your thorough answer.
This has been very useful for me.
I have a question regarding the PBS- why is it necessary for it to be plus Ca++ and Mg++?
I thank you in advance for your help.
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