How can I perform a serine protease phenotypical detection?

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Steingrimur Stefansson

Hi Morteza, the easiest way to detect proteinase activity in these bugs is zymography

http://www.ncbi.nlm.nih.gov/pmc/articles/PMC97462/

http://www.ncbi.nlm.nih.gov/pmc/articles/PMC128331/

Morteza Rabi Nezhad Mousavi

Hi. may you explain more!!! because i dont know zymography . I need method for detection but these articles that you introduced are simple and dont explain efficiently. thanks steingrimur

Hi Morteza, here are some papers on the zymography technique

http://docsdrive.com/pdfs/scienceinternational/sciintl/2013/70-75.pdf

http://www.ncbi.nlm.nih.gov/pubmed/9193704

A list of papers on serine proteinase zymography

http://scholar.google.com/scholar?q=serine+zymography+&btnG=&hl=en&as_sdt=20000005&sciodt=1%2C21&cites=8902318142592596246&scipsc=1