I would like to find an easiest way to detection serine protease activity in Enterococcus faecalis. Thanks.
Hi Morteza, the easiest way to detect proteinase activity in these bugs is zymography
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC97462/
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC128331/
Hi. may you explain more!!! because i dont know zymography . I need method for detection but these articles that you introduced are simple and dont explain efficiently. thanks steingrimur
Hi Morteza, here are some papers on the zymography technique
http://docsdrive.com/pdfs/scienceinternational/sciintl/2013/70-75.pdf
http://www.ncbi.nlm.nih.gov/pubmed/9193704
A list of papers on serine proteinase zymography
http://scholar.google.com/scholar?q=serine+zymography+&btnG=&hl=en&as_sdt=20000005&sciodt=1%2C21&cites=8902318142592596246&scipsc=1
thank's a lot
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